Fungi Flashcards

1
Q

What fungi is depicted?

A

Conidiophore (specifically this is Aspergillus)

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2
Q

What are the Superficial Mycoses Fungi?

A

Malassezia Furfur; Trichosporon

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3
Q

What is the classification of the fungi depicted? What structure is the arrow pointing to?

A

Ascomycetes, Ascus

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4
Q

What is the reproduction of: Zygomycetes, Ascomycetes, Basidiomycetes?

A

Sexual

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5
Q

What stain is being used here? What is the fungi shown?

A

PAS; Candida albicans

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6
Q

What occurs in sexual reproduction before a zygote is formed?

A

Schmoo Phase

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7
Q

What is the action of Triazole drug group?

A

Azoles inhibit the 14 alpha-demethylation of lanosterol to ergosterol without affecting Host cell membrane cholesterol synthesis.

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8
Q
A
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9
Q

What are examples of asexual reproduction?

A

Sporangium, Conidiophore, Arthorospores, yeast

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10
Q
A
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11
Q

What stain is being used? What is depicted? How do you know?

A

Gram Stain, Candida Albicans, Budding is occuring

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12
Q

What phylum/class is Cryptococcus, Malassezia and Trichosporon all apart of?

A

Basidiomycota; Basidiomycetes

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13
Q

How do you classify Histoplasma?

A

Pathogenic - Systemic

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14
Q

What is the identifying structures of Candida albicans?

A

Cells round to oval, pseudohyphae, and some true hyphae with clusters of round blastoconidia at the septa

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15
Q

What fungi is being depicted? What is the morphology of this fungi?

A

Rhizopus; Mold with coenocytic hyphae, spores in sporangium

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16
Q

What fungi is depicted? What structure is unique?

A

Cryptococcus; “Clubbing” basidiospores

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17
Q

How do you classify Aspergillus?

A

Pathogenic - Cutaneous; AND Opportunistic pathogens

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18
Q

What Drug is being used at E? What drug is used at C?

A

C: Azole (Triazole)

E: Amphotericin B

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19
Q

Why is it hard to use gram staining for fungi identification?

A

Everything is gram positive; so it can easily be confused with bacteria

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20
Q

What are examples of Ascomycota Saccharomycetes?

A

Candida, Saccharomyces

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21
Q

How do you classify Candida?

A

Pathogenic - Cutaneous; AND Opportunistic pathogens

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22
Q

What does Malassezia furfur require to grow on agar?

A

Requires Olive oil or some other source of long-chain fatty acids.

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23
Q

What drug inhibits the 14 alpha-demethylation of lanosterol to ergosterol without affecting Host cell membrane cholesterol synthesis?

A

Azole

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24
Q

What stain is being used here? What fungi is present?

A

H & E; Histoplasma Capsulatum

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25
Q

What type of fungi is depicted?

A

Aspergillus

26
Q

What is an example of Ascomycota Eurtiomycetes?

A

Aspergillus

27
Q
A
28
Q

What fungi is depicted?

A

This is the sporulation form Macroconidia (Microsporum Canis)

29
Q

What fungi is depicted?

A

Microsporum Canis

30
Q

What are the common histopathological stains for fungi?

A

H&E, GMS, PAS, Mucicarmine

31
Q

What fungi is identified by: Cells round to oval, pseudohyphae, and some true hyphae with clusters of round blastoconidia at the septa

A

Candida Albicans

32
Q

What is an example of Ascomycota Pneumocystidomycetes?

A

Pneumocystis jirovecii

33
Q

What fungi is being depicted?

A
34
Q

What structure is formed during Meiosis?

A

Zygospore

35
Q

how do you classify Coccidioidies?

A

True Pathogen - System

36
Q

What are examples of Basidiomycota Basidiomycetes fungi?

A

Cryptococcus, Malassezia, Trichosporon

37
Q

What are examples of Sexual reproduction?

A

Zygomycetes, Ascomycetes, Basidiomycetes

38
Q

What is the stain being used? What is the fungi depicted?

A

GMS Stain; Mucor

39
Q

What is the action of Amphotericin B?

A

it is a Polyene (many double bonds) class of drug; it will bind to the Ergosterol in the fungus cell membrane to create artifical pores

40
Q

What are the Opportunistic pathogens in fungi?

A

Aspergillus, Candida, Cryptococcus, Pneumocystis, Zygomycosis

41
Q

Which drug creates pores in the Fungi cell membrane?

A

Amphotericin B

42
Q

What is the stain being used? What is the fungi depicted?

A

GMS & PAS; Cryptococcus

43
Q

What stain is being used? What is the fungi being depicted? What structure is being shown?

A

Lactophenol Cotton Blue; Rhizopus, showing Sporangiophores

44
Q

What is an example of a Glomeromycota Zygomycetes fungi?

A

Rhizopus

45
Q

What should be added to the media when fungi is expected?

A

Brain heart Infusion (BHI); Sabouraud dextrose & BHI (SABHI)

46
Q

What stain is being used? What fungi is being depicted? What disease does it cause?

A

Lactophenol Cotton Blue; Malassezia fur fur - Cutaneous (dandruff; why it requires Olive Oil)

47
Q

What kind of Fungi is depicted?

A

Trichosporon - Arranged into rectangular arthroconidia

48
Q

What is the stain being used? What is the fungi depicted?

A

India Ink, Cryptococcus;

Note the distinguishing Halo

49
Q

What is the stain being used? What is the fungi depicted? What name was given to this famous culture?

A

H & E; Cryptococcus;

50
Q

What type of fungi is Malassezia Furfur?

A

Pathogenic - superficial Mycoses

51
Q

What phylum/class is Rhizopus?

A

Glomeromycota Zygomycetes

52
Q

What are the three fundamental approaches for Identification of Fungi?

A

Microbiological; Histolopathological; Immunological

53
Q

What phylum/class is Aspergillus?

A

Ascomycota Eurtiomycetes

54
Q

What is the classification of the fungi here? What is the structure at the end of the arrow?

A

Zygomycetes; This is a zygospore

55
Q

What is the stain being used? What is the fungi depicted?

A

Mucicarmine; Cryptococcus

*Note the distinguishing Halo

56
Q

What are Candida and Saccharomyces phylum/class?

A

Ascomycota Saccharomycetes

57
Q

What phylum/class is Pneumocystis jirovecii?

A

Ascomycota

58
Q

What are the two true cutaneous pathogens?

A

Candida, Aspergillus

59
Q

What is the reproduction of: Sporangium, Conidiophore, Arthorospores, yeast

A

Asexual

60
Q

What are the common micrososcopy stains for Fungi?

A

Gram stain, Geimsa, Silver, Calcofluor, Lactophenol Cotton Blue