Fungi Flashcards
What fungi is depicted?
Conidiophore (specifically this is Aspergillus)
What are the Superficial Mycoses Fungi?
Malassezia Furfur; Trichosporon
What is the classification of the fungi depicted? What structure is the arrow pointing to?
Ascomycetes, Ascus
What is the reproduction of: Zygomycetes, Ascomycetes, Basidiomycetes?
Sexual
What stain is being used here? What is the fungi shown?
PAS; Candida albicans
What occurs in sexual reproduction before a zygote is formed?
Schmoo Phase
What is the action of Triazole drug group?
Azoles inhibit the 14 alpha-demethylation of lanosterol to ergosterol without affecting Host cell membrane cholesterol synthesis.
What are examples of asexual reproduction?
Sporangium, Conidiophore, Arthorospores, yeast
What stain is being used? What is depicted? How do you know?
Gram Stain, Candida Albicans, Budding is occuring
What phylum/class is Cryptococcus, Malassezia and Trichosporon all apart of?
Basidiomycota; Basidiomycetes
How do you classify Histoplasma?
Pathogenic - Systemic
What is the identifying structures of Candida albicans?
Cells round to oval, pseudohyphae, and some true hyphae with clusters of round blastoconidia at the septa
What fungi is being depicted? What is the morphology of this fungi?
Rhizopus; Mold with coenocytic hyphae, spores in sporangium
What fungi is depicted? What structure is unique?
Cryptococcus; “Clubbing” basidiospores
How do you classify Aspergillus?
Pathogenic - Cutaneous; AND Opportunistic pathogens
What Drug is being used at E? What drug is used at C?
C: Azole (Triazole)
E: Amphotericin B
Why is it hard to use gram staining for fungi identification?
Everything is gram positive; so it can easily be confused with bacteria
What are examples of Ascomycota Saccharomycetes?
Candida, Saccharomyces
How do you classify Candida?
Pathogenic - Cutaneous; AND Opportunistic pathogens
What does Malassezia furfur require to grow on agar?
Requires Olive oil or some other source of long-chain fatty acids.
What drug inhibits the 14 alpha-demethylation of lanosterol to ergosterol without affecting Host cell membrane cholesterol synthesis?
Azole
What stain is being used here? What fungi is present?
H & E; Histoplasma Capsulatum
What type of fungi is depicted?
Aspergillus
What is an example of Ascomycota Eurtiomycetes?
Aspergillus
What fungi is depicted?
This is the sporulation form Macroconidia (Microsporum Canis)
What fungi is depicted?
Microsporum Canis
What are the common histopathological stains for fungi?
H&E, GMS, PAS, Mucicarmine
What fungi is identified by: Cells round to oval, pseudohyphae, and some true hyphae with clusters of round blastoconidia at the septa
Candida Albicans
What is an example of Ascomycota Pneumocystidomycetes?
Pneumocystis jirovecii
What fungi is being depicted?
What structure is formed during Meiosis?
Zygospore
how do you classify Coccidioidies?
True Pathogen - System
What are examples of Basidiomycota Basidiomycetes fungi?
Cryptococcus, Malassezia, Trichosporon
What are examples of Sexual reproduction?
Zygomycetes, Ascomycetes, Basidiomycetes
What is the stain being used? What is the fungi depicted?
GMS Stain; Mucor
What is the action of Amphotericin B?
it is a Polyene (many double bonds) class of drug; it will bind to the Ergosterol in the fungus cell membrane to create artifical pores
What are the Opportunistic pathogens in fungi?
Aspergillus, Candida, Cryptococcus, Pneumocystis, Zygomycosis
Which drug creates pores in the Fungi cell membrane?
Amphotericin B
What is the stain being used? What is the fungi depicted?
GMS & PAS; Cryptococcus
What stain is being used? What is the fungi being depicted? What structure is being shown?
Lactophenol Cotton Blue; Rhizopus, showing Sporangiophores
What is an example of a Glomeromycota Zygomycetes fungi?
Rhizopus
What should be added to the media when fungi is expected?
Brain heart Infusion (BHI); Sabouraud dextrose & BHI (SABHI)
What stain is being used? What fungi is being depicted? What disease does it cause?
Lactophenol Cotton Blue; Malassezia fur fur - Cutaneous (dandruff; why it requires Olive Oil)
What kind of Fungi is depicted?
Trichosporon - Arranged into rectangular arthroconidia
What is the stain being used? What is the fungi depicted?
India Ink, Cryptococcus;
Note the distinguishing Halo
What is the stain being used? What is the fungi depicted? What name was given to this famous culture?
H & E; Cryptococcus;
What type of fungi is Malassezia Furfur?
Pathogenic - superficial Mycoses
What phylum/class is Rhizopus?
Glomeromycota Zygomycetes
What are the three fundamental approaches for Identification of Fungi?
Microbiological; Histolopathological; Immunological
What phylum/class is Aspergillus?
Ascomycota Eurtiomycetes
What is the classification of the fungi here? What is the structure at the end of the arrow?
Zygomycetes; This is a zygospore
What is the stain being used? What is the fungi depicted?
Mucicarmine; Cryptococcus
*Note the distinguishing Halo
What are Candida and Saccharomyces phylum/class?
Ascomycota Saccharomycetes
What phylum/class is Pneumocystis jirovecii?
Ascomycota
What are the two true cutaneous pathogens?
Candida, Aspergillus
What is the reproduction of: Sporangium, Conidiophore, Arthorospores, yeast
Asexual
What are the common micrososcopy stains for Fungi?
Gram stain, Geimsa, Silver, Calcofluor, Lactophenol Cotton Blue
