Foundations 3

Question 1

What are nuclear localization signals and what are they composed of?

Answer 1

Highly conserved 4-8 amino acid sequences rich in lysine, arginine, and proline (positively charged)

Question 2

What regulates transition from G1 to S phase of the cell cycle?

Answer 2

P53 (Li-Fraumeni) and Rb (osteosarcoma and retinoblastomas)

Question 3

How does p53 regulate the cell cycle?

Answer 3

Controls activation of p21 which then binds to and inactivates Cyclin-CDK complexes

Question 4

Which amino acids can be modified by the Golgi?

Answer 4

Serine, Threonine (o-oligosaccharide), and asparagine (n-oligosaccharide)

Question 5

What addition to proteins by the Golgi targets them to lysosomes?

Answer 5

Mannose-6-phosphate

Question 6

Which Cyclin complexes cause progression from G1-S?

Answer 6

Cyclin D/CDK4

Cyclin E/CDK2

Question 7

Which Cyclin complexes cause progression from G2-M?

Answer 7

Cyclin A/CDK2 (Mitotic Prophase)

Cyclin B/CDK1 (Lamin Breakdown)