Formation of Acetyl CoA from pyruvate?

Question 1

NAME
E1
E2
E3

Answer 1

E1: pyruvate decarboxylase
E2: Lipoaceytle transferase
E3: Dihydrolipoamide DH

Question 2

Starting with pyruvate describe the first step involving E1

Answer 2

E1= Pyruvate decarboxylase

• Pyruvate decarboxylated to form the C2 unit
• C2 unit accepted by TPP-H cofactor

Question 3

Starting with TPP- C2 describe the next step involving enzyme 2

Answer 3

E2= Lipoacetyle transferase

• lipoyl group accepts C2 unit
• acetyle CoA formation
• regeneration of TPP-H

Question 4

STEP 3 of the formation of Acetyle coA

Answer 4

E3- dihydrolipoamide dh

• oxidation of lipoyl group
• FAD, subunits of E3 histidine and disulphide bridge. Reduction of one sulfur (SH) and left without (s-). His also reduced
• Oxidation of NADH+ H+ to form NAD+ allows for reduction in enzyme residues

Question 5

TPP stands for

Answer 5

thyamine pyrophosphate

Question 6

FAD stands for

Answer 6

Flavin adenine Dinucleotide

Question 7

LIP

Answer 7

lipoid acid

Question 8

Net gain of ATP after this process?

Answer 8

2ATP

Question 9

Anaerobic respiration in humans to form Lactate is accompanied by?

Answer 9

NADH + H+ to form NAD

process purpose is to regenerate NAD+
plus a reduction reaction which indicates NAD must be involved

Question 10

yeast 2 step in anaerobic respiration to form what

Answer 10

Pyruvate, decarboxylation to form ethanal, which is reduced to form ethanol

Question 11

How does the E2 enzyme enable communication between all residues within the complex

Answer 11

Long swinging arm.

lysine residue and lipoid acid residue

Question 12

In ecoli structure of PDHC

Answer 12

E1:48 E2:24 E3:24

cuboidal structure
4x6= 24 (e2) rest surrond

Question 13

In mammals PDHC

Answer 13

60 COPIES

pentagonal dodecahedron