Forensic Analysis of Other Body Fluids

Question 1

Definition of Saliva

Answer 1

Mostly water with small amounts of electrolytes, proteins, antibodies, and enzymes

Question 2

Definition of Amylase

Answer 2

An enzyme that breaks down complex carbohydrates into smaller sugar molecules

Question 3

What are the 2 types of amylases?

Answer 3

1. B-Amylases
2. a-Amylases

Question 4

What Presumptive Tests are used for Saliva?

Answer 4

-Visual/UV light (for any fluorescing stains)
-Histological (buccal epithelial cells)
-Starch iodine
-Phadebas
-Saligae

Question 5

What Confirmatory Test is used for Saliva?

Answer 5

ELISA
“Enzyme-Linked Immunosorbent Assays”

Question 6

What are the 3 processes in urine formation?

Answer 6

1. Filtration
2. Reabsorption
3. Secretion

Question 7

What Presumptive Tests are used for Urine?

Answer 7

-Visual (yellow stain/distinctive smell)
-ALS
-DMAC
-Urease assays
-Jaffe

Question 8

What Confirmatory Tests are used for Urine?

Answer 8

-RSID Urine (Rapid Stain Identification)
-ELIZA (Enzyme Linked Immunoabsorbent Assay)
-LCMS (Liquid Chromatography Mass Spectrometry)

Question 9

Definition of Feces

Answer 9

End product after digestion that is made up of undigested food, mucosal cells, and bacteria

Question 10

What is the Schlesinger Test?

Answer 10

Sample mixed w/ saturated zinc acetate in ethanol

Question 11

What is the Edelman Test?

Answer 11

Sample mixed w/ a mercuric salt to yield a pink colored compound

Question 12

Definition of Menstruation

Answer 12

The periodic discharge of blood and the elimination of the degenerated lining of the endometrium from the uterus of non-pregnant women

Question 13

What is Lactate Dehydrogenase? (LDH)

Answer 13

An enzyme that plays an important role in glycolysis