Emerging Techniques in Body Fluid Identification Flashcards
1
Q
What are the two types of Spectroscopic Techniques?
A
- Infrared Spectroscopy
- Raman Spectroscopy
2
Q
What is Infrared Spectroscopy?
A
A form of vibrational spectroscopy
(can ID body fluids, chemical substances, drugs, etc.)
3
Q
What is Raman Scattering?
A
A photon that causes molecules to gain vibrational energy
4
Q
What are the advantages of Raman Scattering?
A
-Minimal preparation required
-Non-destructive method
-Fast
-Cost Effective
5
Q
What are the 3 Molecular Techniques in Body Fluid ID?
A
- DNA Methylation
- messengerRNA
- microRNA
6
Q
What is Epigenetics?
A
Involves any changes to the DNA that does not effect the sequence
7
Q
The central dogma includes 3 processes…
A
- Replication: copies the genetic info. of a cell
- Transcription: converts a gene into a single stranded RNA molecule
- Translation: the genetic info is carried in mRNA
8
Q
messengerRNA is…
A
A single stranded nucleic acid
9
Q
messengerRNA consists of 4 kinds of nucleotides…
A
- Uracil
- Guanine
- Adenine
- Cytosine
