Follicles and CL Flashcards
Function of ovary
-develop female oocyte for fertilization
-produce and secrete sex steroid hormones that regulate reproductive health
Ovary structure
-has 2 regions: outer cortex and inner medulla (opposite in horses)
-cortex has ovarian follicles
-medulla has blood vessels, nerves, connective tissue
Folliculogenesis
The developmental process of ovarian follicles starting from a reserve of quiescent primordial follicles set up in early life and ending with ovulation OR atresia (follicle death)
Stages of follicles
- Primordial
- Primary
- Early secondary
- Secondary
- Antral
- Preovulatory
Follicle phases
- Resting phase (primordial follicles)
- Growth phase
Gonad development stages
Initially, Bipotential gonads meaning they can become either the testes or ovary
Then if cellular events occur correctly, will become ovary or testes
Gonad transformation events needed to become ovary
- supporting cells become granulosa cells
- steroidogenic cell precursors become theca cells
- Primordial germ cells become oocytes through oogenesis
Where does development of ovary occur?
Starts at the mesonephric surface epithelium in the location of the future gonadal ridge
Development steps of primordial follicles
- Development of the ovary starts at mesonephric surface epithelium
- Mesonephric surface epithelial cells change phenotype into gonadal ridge epithelial-like (GREL) cells
3.GREL cells proliferated, basal lamina underneath breaks down allowing stromal cells to penetrate into gonadal ridge - GREL cells continue proliferation, and primordial germ cells migrate to ridge between them
- Oogonia proliferate and stroma penetrates towards the ovarian surface, enclosing the oogonia and GREL cells into ovigerous cords
- Compartmentalization occurs in cortex and medulla. GREL cells at surface align to basal lamina and start differentiation into typical ovarian surface epithelium
- Ovigerous cords are partitioned into smaller cords and eventually follicles which contain GREL cells forming granulosa cells and oogonia= oocytes
- Surface epithelium becomes single layered and tunica albuginea
Primordial follicle structure
-consist of primary oocyte, surrounded by single layer of flat follicular cells
Distribution of primordial follicles in ruminants vs carnivores
Ruminants: equally distributed
Carnivores: follicles in clusters
Primary follicles
-Epithelium is cuboidal
Secondary follicles
A follicle with 2-6 layers of granulosa cells
-also have additional somatic theca cell layer (steroid producing)
-also has initial deposition of zona pellucida material (glycoprotein layer secreted by oocyte and granulosa cell)
Tertiary (antral) follicle
Follicles with more than 6 layers of granulosa cells and a fluid filled antrum
-Will have fully developed zona pellucida, development of internal and external theca cell layers, and cumulus cell formation
Graafian (preovulatory) follicles
-most mature stage
-granulosa cells are producing estradiol