Focus on Microbiology Flashcards

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1
Q

What are the two main types of cells discussed in the lecture?

A

Prokaryotic and eukaryotic cells

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2
Q

What is the primary focus of the lecture?

A

Bacteria

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3
Q

True or False: Most bacteria cause serious problems to humans.

A

False

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4
Q

What is taxonomy?

A

The classification and naming of living organisms

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5
Q

What are the two parts of a bacterial name?

A

Genus and species

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6
Q

What is the full name of E. coli?

A

Escherichia coli

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7
Q

In bacterial nomenclature, which part is written first?

A

Genus

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8
Q

What are the three domains of life according to Carl Woese?

A

Bacteria, archaea, eukaryota

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9
Q

What defines eukaryotic cells?

A

Contain a nucleus

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10
Q

What defines prokaryotic cells?

A

Do not contain a nucleus

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11
Q

What size range do bacteria typically fall within?

A

0.2 to 2 µm in diameter and 1-8 µm in length

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12
Q

What are the main components of a bacterial cell?

A
  • Cell membrane
  • Cytoplasm
  • Cell wall (peptidoglycan)
  • Circular DNA
  • Plasmids
  • Ribosomes
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13
Q

What is the process by which bacteria replicate?

A

Binary fission

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14
Q

Fill in the blank: Bacteria can be classified according to _______.

A

Morphology, mode of nutrition, pathogenicity

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15
Q

What type of bacteria are cocci?

A

Round cells

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16
Q

What are bacilli?

A

Rod-shaped bacteria

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17
Q

What are spirilla?

A

Corkscrew-shaped bacteria

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18
Q

What are vibrios?

A

Comma-shaped bacteria

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19
Q

What is the significance of extremophiles?

A

They thrive in extreme conditions

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20
Q

What is the singular form of cocci?

A

Coccus

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21
Q

What type of bacteria causes cholera?

A

Vibrio cholerae

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22
Q

What is the primary characteristic of archaea?

A

They are prokaryotic and often extremophiles

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23
Q

What are the different types of extremophiles based on environmental preferences?

A
  • Thermophiles (high temperature)
  • Psychrophiles (low temperature)
  • Barophiles (high pressure)
  • Acidophiles (acidic environment)
  • Alkaliphiles (alkaline environment)
  • Halophiles (high salt)
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24
Q

True or False: Bacteria have membrane-bound organelles.

A

False

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25
Q

What is the study of fungi called?

A

Mycology

Mycology is the branch of biology concerned with the study of fungi, including their genetic and biochemical properties.

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26
Q

What domain of life do fungi belong to?

A

Eukaryota

Fungi are classified under the domain Eukaryota, which includes organisms with complex cells.

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27
Q

What are the two main types of fungi based on structure?

A
  • Unicellular yeasts
  • Multicellular molds

Fungi can exist in various forms, with yeasts being unicellular and molds being multicellular.

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28
Q

What type of cells are fungi classified as?

A

Eukaryotic cells

Eukaryotic cells contain a nucleus and membrane-bound organelles.

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29
Q

What is the network of hyphae called?

A

Mycelium

Mycelium is formed by a mass of hyphae and is often visible to the naked eye.

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30
Q

What is the reproductive structure of fungi from which spores can be released?

A

Sporangia

Sporangia are structures that produce and contain spores in fungi.

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31
Q

How do yeasts reproduce?

A

Budding

In budding, a parent cell forms a bud that eventually detaches to become a new organism.

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32
Q

What type of nutrition do fungi primarily exhibit?

A

Decomposers

Fungi play a crucial role in ecosystems by breaking down dead matter.

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33
Q

What are the two types of spores produced by molds?

A
  • Asexual spores
  • Sexual spores

Asexual spores are formed from one organism, while sexual spores result from the fusion of cells from two organisms.

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34
Q

Fill in the blank: Fungi can be classified into four main groups: Chytridomycota, Basidiomycota, ________, and Zygomycota.

A

Ascomycota

These classifications are based on specific characteristics and reproductive methods of the fungi.

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35
Q

True or False: Fungi can only reproduce asexually.

A

False

Fungi can reproduce both asexually and sexually.

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36
Q

What are hyphae?

A

Long tubular thread-like filaments

Hyphae are the basic structural units of fungi and can grow to large sizes.

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37
Q

What is the difference between septate and aseptate hyphae?

A
  • Septate: have cell walls (septa)
  • Aseptate: do not have cell walls

The presence or absence of septa affects the structure and function of the hyphae.

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38
Q

What are protozoa?

A

Unicellular eukaryotes that can be free-living or parasitic

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39
Q

What is the size range of protozoa?

A

From 1 micrometer to several millimeters or more

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40
Q

What type of environments do protozoa generally inhabit?

A

Moist environments such as fresh water, marine environments, and soil

41
Q

How do protozoa obtain their food?

A

They are heterotrophic and cannot synthesize their own food

42
Q

What are the eight phyla in the kingdom protozoa?

A
  • Euglenozoa
  • Amoebozoa
  • Metamonada
  • Choanozoa
  • Loukozoa
  • Percolozoa
  • Microsporidia
  • Sulcozoa
43
Q

What is the most well-known mode of feeding for protozoa?

A

Phagocytosis

44
Q

What structure do Amoeba use to engulf their prey?

A

Pseudopodia

45
Q

What is osmotrophy?

A

A process where nutrients are absorbed through cell membranes

46
Q

What are the two main modes of locomotion for protozoa?

A
  • Pseudopodia
  • Cilia
  • Flagella
47
Q

How do protozoa reproduce asexually?

A

By binary fission

48
Q

What is a dormant stage that some protozoa can enter?

A

Cysts

49
Q

What is the proliferative phase of protozoa?

A

The active phase where they feed and reproduce

50
Q

Name a disease caused by Plasmodium falciparum.

A

Malaria

51
Q

What is the causative agent of Giardiasis?

A

Giardia duodenalis

52
Q

True or False: All protozoa are microscopic.

A

False

53
Q

What is the term for the mouth-like opening in some protozoa?

A

Cytostome

54
Q

Fill in the blank: Protozoa are classified as _______ eukaryotes.

A

unicellular

55
Q

What is the role of protozoa in ecosystems?

A

They are an important part of many ecosystems

56
Q

What type of protozoa help wood-eating termites digest complex sugars?

A

Symbiotic protozoa

57
Q

What is the term for protozoa that can survive in harsh conditions by forming cysts?

A

Dormant cyst stage

58
Q

Name an example of a protozoan that causes primary amoebic meningoencephalitis.

A

Naegleria fowleri

59
Q

What are viruses considered in terms of their classification in the tree of life?

A

Viruses are not considered living things because they cannot reproduce independently.

60
Q

What is the primary structure of a virus composed of?

A

Nucleic acid surrounded by a protein coat.

61
Q

What types of nucleic acid can be found in viruses?

A

DNA or RNA, but not both.

62
Q

What term is used to describe viruses that require a host’s cellular machinery to reproduce?

A

Obligate intracellular parasites.

63
Q

What is the size range of viruses in nanometers?

A

20-300 nm.

64
Q

What is a bacteriophage?

A

A virus that infects bacteria.

65
Q

What is the protein coat of a virus called?

A

Capsid.

66
Q

What term refers to the complete virus particle, including its protein coat and envelope (if present)?

A

Virion.

67
Q

What does cell tropism refer to?

A

The limited range of cell types that can support the reproduction of a particular virus.

68
Q

What is a zoonosis?

A

An infectious disease caused by a pathogen that has jumped from a non-human animal to a human.

69
Q

What is the primary reason why scientists did not identify viruses until the 20th century?

A

The development of the electron microscope and enhanced analytical techniques.

70
Q

What are the two types of proteins encoded by the viral genome?

A
  • Structural proteins
  • Non-structural proteins
71
Q

What major role do viruses play in disease?

A

They can destroy host cells, leading to disease symptoms.

72
Q

What is reverse transcriptase?

A

An enzyme that synthesizes a complementary DNA strand from RNA.

73
Q

What type of virus is HIV classified as?

A

Retrovirus.

74
Q

Fill in the blank: The disease caused by the novel coronavirus is known as _______.

A

Covid-19.

75
Q

True or False: Viruses can be visualized using a light microscope.

A

False.

76
Q

What do the terms ‘structural’ and ‘non-structural’ proteins refer to in the context of viruses?

A
  • Structural proteins: used to construct the virion
  • Non-structural proteins: often enzymes involved in attachment and replication
77
Q

What is the primary cause of the symptoms associated with viral infections?

A

The damage and destruction of host cells.

78
Q

What is the typical diameter of viruses in comparison to bacteria?

A

Viruses are approximately 100 times smaller than bacteria.

79
Q

What is the significance of the Chamberland-Pasteur filter in virology?

A

It was used to demonstrate that disease could be transmitted even when bacteria were removed from a sample.

80
Q

What is the main component of the viral envelope?

A

Lipoprotein or glycoprotein.

81
Q

What is the general range of genome sizes for viruses?

A

A few thousand nucleotides to 250,000 nucleotides.

82
Q

What does the term ‘obligate intracellular parasites’ imply about viruses?

A

They depend on living host cells for replication.

83
Q

What kind of microscopy is necessary to visualize viruses?

A

Electron microscopy.

84
Q

What is the primary function of light microscopy?

A

To use visible light to observe specimens

Light microscopy allows examination of whole microorganisms and their fine details.

85
Q

What are the main components of a light microscope?

A

A series of lenses and a light source

Light rays from an illuminator pass through a condenser, then to the objective lenses, and finally to the ocular lens.

86
Q

How is total magnification calculated in a compound microscope?

A

By multiplying the objective lens magnification by the ocular lens magnification

Most objective lenses come in magnifications such as 10X, 40X, and 100X.

87
Q

What is the highest resolution possible with a compound light microscope?

A

Approximately 0.2µm

Resolution is often the limiting factor for visualizing microorganisms rather than magnification.

88
Q

What is the purpose of immersion oil in microscopy?

A

To improve the resolving power of the lenses

The oil has the same refractive index as glass, preserving the direction of light rays.

89
Q

What technique is used to enhance contrast in specimens under a microscope?

A

Staining

Staining changes the refractive index of specimens, allowing for sharper images.

90
Q

What is the Gram staining technique used for?

A

To distinguish between gram-positive and gram-negative bacteria

Crystal violet dye binds more tightly to gram-positive cells due to their thick peptidoglycan layer.

91
Q

What color do gram-positive bacteria appear after Gram staining?

A

Purple

Gram-negative bacteria can be counterstained with safranin, appearing pink.

92
Q

Who first described phase contrast microscopy?

A

Frits Zernike

He introduced this technique in the 1930s.

93
Q

What does phase contrast microscopy exploit?

A

The difference in refractive index between cells and their surroundings

This creates a phase difference between light passing through the specimen and surrounding medium.

94
Q

What is one advantage of dark-field microscopy?

A

Superior resolution compared to bright-field or phase contrast microscopy

It is effective for observing microorganisms with flagella.

95
Q

True or False: The highest magnification for light microscopes is approximately 2000X.

A

True

Beyond this magnification, resolution tends not to improve.

96
Q

Fill in the blank: The _______ lens is closest to the specimen in a light microscope.

A

[objective]

The ocular lens is the eyepiece that further magnifies the image.

97
Q

What is the role of a phase plate in phase contrast microscopy?

A

To increase the contrast of the image produced

It enhances the visibility of the specimen against its background.

98
Q

What type of microscopy results in a light specimen on a dark background?

A

Dark-field microscopy

This technique is useful for high-contrast images of specimens.

99
Q

What must be done to obtain a clear image under a compound light microscope?

A

Specimens must contrast sharply with their medium

Changing the refractive index of specimens through staining helps achieve this contrast.