Fluorescent Proteins

Question 1

What is Bioluminescence?

Answer 1

The production and emission of light by a living organism

Question 2

What colour is Aequorin?

Answer 2

Blue

Question 3

What colour is GFP?

Answer 3

Green

Question 4

What ion will cause Aequorin to fluoresce?

Answer 4

Ca2+

Question 5

What wavelength does Aequorin emit?

Answer 5

470nm

Question 6

What will cause GFP to fluoresce?

Answer 6

Absorption of blue light

Question 7

What wavelength does GFP emit?

Answer 7

510nm

Question 8

How many amino acids does GFP have?

Answer 8

238

Question 9

What shape is GFP?

Answer 9

Cylinder shaped
* central α-helix
* 11 anti-parallel β-sheets

Question 10

What wavelength does GFP absorb light at?

Answer 10

475nm

Question 11

Which amino acids does GFP fluorescence originate from?

Answer 11

• Serine
• Tyrosine
• Glycine

Ser65 - Tyr66 - Gly67

Question 12

What happens to Tyr66 on GFP during post-translational modification?

Answer 12

Oxidation

Question 13

What is the crucial step in the post-translational modification of GFP?

Answer 13

Attack of backbone NH from Gly67 onto amide bond of Ser65

Question 14

Which GFP variant improves spectral properties?

Answer 14

S65T

Question 15

Which GFP variant improves folding?

Answer 15

F64L
(Phenylalanine to Leucine)