Centrifugation & Sedimentation Techniques Flashcards
What is the equation for the Sedimentation Coefficient, s?
How does the MASS of a particle affect the Sedimentation Coefficient?
The larger the mass, the GREATER the sedimentation coefficient and the greater the sedimentation speed
How does the SHAPE of a particle affect the Sedimentation Coefficient?
The more spherical, the LOWER the f value, and the HIGHER the sedimentation coefficient
What are the TWO main benefits of Fixed-Angle centrifugation opposed to Swinging Bucket?
- Better Separation
- Shorter Run Time
What is RCF?
Relative Centrifugal Force
What is the equation for RCF?
How are molecules separated during Moving Boundary Centrifugation (differential velocity)?
Molecules will precipitate at the bottom in distinct layers
How are molecules separated during Moving Zone Centrifugation?
- Sample is applied on top of more dense solvent
- Molecules are separated through a density gradient according to size, shape, and density
What reagent is added to a sample in Blood Cell Fractionation?
Ficoll-Paque Plus
What is Ultra-Centrifugation used for?
- Analytical technique to study purified proteins
- Preparative isolations of NAs
What centrifugation speed does Ultra-Centrifugation use?
Up to 60,000 rpm OR 200,000 G
What is required in Ultra-Centrifugation to stop the sample from over-heating?
Vacuum
