Fluorescence Microscopy quiz Flashcards
1
Q
What is excitation (incident) light
A
occurs at smaller wavelength and higher energy, focused on specimen which absorbs and re-radiates the light
2
Q
What is emission (fluorescent) light
A
higher wavelength and lower energy; opposite of excitation light
3
Q
What is the purpose of fluorophores/fluorochrome antibody labels
A
absorb light from incident light and convert to longer wavelength and lower energy
4
Q
What is a conventional microscope
A
produces magnified image
5
Q
What is a fluorescence microscope
A
illuminates sample using higher intensity light
6
Q
What is photoluminescence
A
when specimens absorb and re radiate light
7
Q
What is Epifluorescence
A
combination of excitation and emission wavelengths travel through specimen emitting fluorescence
