Flow Cytometry Webcast Flashcards
Antigen: CD3
Cellular Expression: T cells
Antigen: CD4
Cellular Expression: Helper T cells
Antigen: CD8
Cellular Expression: Cytotoxic T cells
Antigen: CD13
Cellular Expression: Granulocytes, Monocytes
Antigen: CD14
Cellular Expression: Monocytes
Antigen: CD15
Cellular Expression: Granulocytes, Monocytes
Antigen: CD19
Cellular Expression: B cells
Antigen: CD20
Cellular Expression: B cells
Antigen: CD34
Cellular Expression: Blasts/Stem Cells
Antigen: CD45
Cellular Expression: Leukocytes
What does forward scatter tell you?
It reflects the size of the cell (x-axis)
What does side scatter tell you?
It reflects the complexity of the cell cytoplasm (y-axis)
What is immunophenotype aberrancy?
Inappropriate expression of an antigen