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Serology > Flow Cytometry and Cellular Markers > Flashcards

Flow Cytometry and Cellular Markers Flashcards

1
Q

CD3

A

Most specific T cell Ag, stains Th/Tc/NKT

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2
Q

CD4

A

Th cells, normally at a higher percentage than Tc cells, although this is reversed in HIV patients

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3
Q

CD5

A

Unique to CLL B cells, otherwise only on T cells

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4
Q

CD7

A

Found on T-cells, used as a marker for mycosis fungoides (Sézary Syndrome)

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5
Q

CD8

A

Tc cell marker

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6
Q

CD10

A

CALLA Found on young B cells, used to aid diagnoses of: ALL, Angioimmunoblastic T cell lymphoma, Burkitt’s lymphoma, Diffuse large cell lymphoma, and follicular lymphoma

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7
Q

CD11c

A

Marker for M4/5, and Hairy Cell Leukemia

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8
Q

CD13

A

Myeloid Ag, associated with AML diagnosis (M1-6) and CML

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9
Q

CD14

A

Monocyte differentiation Ag

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10
Q

CD16a

A

Binds Fc portion of IgG, markers NK cells and Macrophages

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11
Q

CD19

A

Earliest B cell Ag, more sensitive to detecting ALLs than CD20

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12
Q

CD20

A

Appears between CD19/10 and CD21/22 on B cells, used for more mature B cell lymphomas

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13
Q

CD22

A

Pan B cell marker

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14
Q

CD23

A

Promotes B cell differentiation to Plasma cells, differentiates CLL/SLL

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15
Q

CD25

A

Regulatory T cell marker, associated with advanced T cell cutaneous lymphoma

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16
Q

CD33

A

Classic Myeloid Ag in AMLs and CMLs

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17
Q

CD34

A

Marker for stem cells (blasts), in ALLs and AMLs

18
Q

CD38

A

Plasma cell marker

19
Q

CD45

A

Pan leukocyte Ag

20
Q

CD56

A

NK cell marker

21
Q

CD117

A

Stem cell marker

22
Q

CD2

A

Early T marker Can indicate T-ALL

23
Q

Naive T cells

A

Express L-selectin and lack CD25, CD44, and CD69 Gain these markers plus CD62 when activated

24
Q

MHC Class I

A

Intercellular Ag presentation, only recognized by Tc cells (CD8) that kill the cells to which they bind

25
Q

MHC Class II

A

Present extracellular Ag, and only bind to Th (CD4) cells

26
Q

Sarcoidosis

A

Tiny clumps of inflammatory cells (granulomas) triggered by Th lymphocytes causing lumps to form on skin and potentially in lungs

27
Q

Markers tested by Flow Cytometry

A
  1. Leukocyte markers
  2. Myeloperoxidase
  3. T-helper cells, T suppressor cells
  4. T-cells, B-cells, NK cells
  5. Terminal Deoxynucleotidyl Transferase (TDT)
28
Q

AML Markers

A

MPO, CD13, CD33 should be present without Lymphoid markers, excluding CD7 and TdT

CD34 and CD7 are associated with immature cells rather than cell lineage

29
Q

AML-M3

A

Characterized by lacking HLA-DR while expressing the other AML markers

30
Q

AML-M4/M5

A

Show simultaneous expression of CD14 and CD64

31
Q

B-cell ALL

A

CD22 with CD19 and HLA-DR CD22 can appear dimly in AML, so the simultaneous expression of all 3 is required

32
Q

T-cell ALL

A

CD3 with CD7 Subtypes can be defined by the following markers: CD1a, CD2, CD3, CD4, CD8

33
Q

B-cell Non-Hodgkin Lymphoma

A

Clonal expression of kappa/lambda light chains with expression of pan B cell Ag (CD19)

34
Q

Plasmocytoma

A

CD38 (or CD138) without B cell specific Ag or surface Ig

35
Q

Hairy Cell Leukemia

A

High forward scatter, CD11c and CD103

36
Q

B-cell CLL

A

CD19, CD20, CD5, CD23, CD22 and low surface Ig levels combine to indicate CLL Lack CALLA (CD10)

37
Q

Interpreting Flow Cytometry Data

A

Ag expression should be analyzed without selecting analysis regions in case the dot plot goes off the scale Dividing them later based on light scatter with different dyes allows the data to be interpreted more easily

38
Q

T-cell CLL

A

CD3 (pan T-cell), CD4 (Th)

39
Q

NKT Cells

A

CD2, CD16, CD56 while lacking CD3 and CD4

40
Q

Sézary Syndrome

A

Th cells lack CD7

41
Q

Differentiating Young and Mature B cells

A

CD19 marks all B cells and appears before CD10, CD20 (doesn’t appear on young B cells), CD22, CD23

Serology (8 decks)

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