Find it, Lose it, Move it

Question 1

a systematic approach to performing experiments in developmental biology in shedding light to the specific molecules or structures that affect the organism’s functions as it develops.

Answer 1

Find it, Lose it, Move it

Question 2

pertains to knowing the correlation of a molecule or structure with a function

Answer 2

Find it

Question 3

when a function is lost when the molecule or structure is also lost by negative inference be it by mutation or by inhibition

Answer 3

Lose it

Question 4

putting the molecule or structure in a different place which it isn’t commonly found and assess whether the function is still evident (positive inference)

Answer 4

Move it

Question 4

The part of the sperm that attaches to the egg is the

Answer 4

acrosome

Question 5

Inhibiting _____ inhibits sperm-egg adhesion.

Answer 5

bindin

Question 6

In mammalian sperms, _____ is found in the acrosome which binds to the egg cell membrane.

Answer 6

Izumo

Question 7

is found on the egg cell and is responsible for binding to Izumo protein.

Answer 7

Juno

Question 8

How can we be certain that we have identified the correct function of the structure or molecule when there are phenotypes that can be expressed by different molecules or structures that serve the same function as in the redundancy of biological systems honed by mutation, evolution, and natural selection?

Answer 8

Using double knockouts
Compensatory Mechanisms
Evolutionary and Comparative Biology
Phenotypic Rescue
Systems Biology Approach (-omics)

Question 9

Are there instances where the concept of “Find it, Lose it, Move it” cannot be utilized in an experimental study?

Answer 9

If the target molecule or structure is essential for survival, completely removing it (Lose it) may cause lethality before its function can be studied.

Redundancy in biological systems means that losing one component may not produce a detectable phenotype due to compensatory mechanisms.

Question 10

In “Move it”, what are the factors or criteria to be considered to relocate the molecule or structure of interest or can we just place them in any cell?

Answer 10

Cellular and Tissue Context
Spatiotemporal Compatibility
Dosage and Concentration
Post-Translational Modifications and Processing
Interaction with Other Molecular Players