Final Review Flashcards
what are the 3 things the CTD is involved in w/ RNA processing
5’ capping, splicing, polyadenylation
EF-Tu in prokaryotes
brings charged tRNA to the A site to be joined with the polypetide chain at the P site
(Tu = T for taxi)
EF-G in prokaryotes
bonds to the A site to make the tRNA move to the E and P sites
(G = get over there)
PTC
peptidal transferase center - makes peptide bonds at P site
eIF1a
same as EF-G - blocks the A site
(1a = first - gotta block the A site first)
eIF1 and eIF3
eIF6
same as IF3 - prevents ribosomal subunits from joining too early
(1 and 3 = 13 = too young for the ribosomes to join!)
eIF2 and eIF5
same as EF-Tu - bring initiator tRNA to the A site
(2 and 5 = $25 = cheap uber)
eIF5b
brings ribosomal subunits together
(b = brings) (5 = together 5ever)
subunits of eIF4
eIF4A, eIF4G, eIF4E
eIF4A
ATPase activity, helicase activity
(A for ATP, 4 for helicase - 4 has the same vibes as H?)
eIF4G
interacts with polyA binding proteins
eIF4
capping complex
eIF4E
binds to cap
Kozak’s sequence
eukaryotic initiation site
RCCAUGG (R is A or G)
ternary complex
made up of small ribosomal subunit, eIF3, eIF2, metTRNAi
binds to eIF1a
Shine-Dalgarno sequence
AGGAGGU
ribosome binding site
where is the shine-dalgarno sequence
in the 5’ UTR
IF1
blocks the A site
IF2
has GTPase activity which gives it energy to deliver the fMet-tRNA to P site and complete the preinitiation complex
IF3
binds to the E site - keeps large and small ribosomes apart
RF1 recognizes these stop codons
UAA, UAG
RF2 recognizes these stop codons
UAA, UGA
prokaryotic genes are polycistronic
one gene produces multiple, functionally related proteins
