Final (Lecture) Flashcards
What are the 6 steps in plant breeding?
- Identify Plants and Traits
- Sources of Variation
- Plant Modes of Reproduction
- Breeding Strategies
- Trials
- Marketing
What is a trait?
a visual or measurable (usually variable) characteristic of an organism or population
DNA to protein to visible trait
What is germplasm?
any source of genetic material
What are the 2 options of germplasm and what do they capitalize on?
- Find material (variation, chance seedling)
- Create material (radiation, UV, chemicals)
Mutations
What is the center of diversity?
region where extensive genetic variation in a species exists
What is the center of origin?
region where species originated
What are two ways to create variation?
mutation breeding and genetic engineering
What is mutation breeding?
induce mutations to create variation: UV light, ethyl methanesulfonate, radiation
What is genetic engineering and what is used?
taking genes from one species and inserting into another; agrobacterium
What is transformation? (transgenic plants)
non-sexual process by which a trait is introduced from a different species into a plant (gene gun, mostly Agrobacterium)
What is horizontal gene transfer?
transfer of genes between organisms
What is transgenic?
organism contains DNA that has been artificially introduced
How does the gene get into plants? (making a transgenic)
agrobacterium transformation
What is agrobacterium transformation?
natural genetic engineer that causes galls or tumors in plants to enhance bacterial growth
Ti plasmid removes unwanted DNA and inserts desired ones
What is gene construct and what are the components (3)?
DNA segment designed to be functional when it is inserted into plant DNA
Promoter- help gene be read
Coding sequence- gene of interest to protein to trait
Termination sequence- stops the gene being read
What are the sex expressions within a flower?
stamen (male), pistil (female), or both
What are the sex expressions within a plant?
male, female, or both
What are the methods of pollination? (2)
self pollination- pollen fertilizes egg of same individual
cross pollination- pollen exchanges between 2 plants
What are the breeding strategies? (4)
mass selection
hybridization
back crossing
manipulating ploidy
What is mass selection? (breeding strategy)
selection that increases
desirable trait in a population
What is hybridization? (breeding strategy)
a cross between two sources of
germplasm
Same species or close family members
Often, hybrid
vigor present
What is back crossing? (breeding strategy)
movement of a desirable trait
into a plant/cultivar deficient of that trait
after 6 generations will be 99%
elite / 1 % “improved” genetics
What is manipulating ploidy (polyploidy)? (breeding strategy)
number of
chromosomes in a cell
* Higher ploidy levels: larger cells, larger leaves,
thicker leaves, larger flowers, etc.
* Colchicine stops spindle fibers → doubles DNA
What is a trial?
location for the evaluation of cultivars