Final - Definitions Ch 14

Question 1

Sequence assembly

Answer 1

The compilation of thousands or millions of independent DNA sequence reads into a set of contigs and scaffolds

Question 2

Consensus sequence

Answer 2

The nucleotide or amino acid sequence of a nucleic acid or protein, respectively, that is derived by aligning similar sequences and determining the most common nucleotide or amino acid at each position

Question 3

Whole-genome shotgun (WGS) sequencing

Answer 3

A strategy for obtaining the sequence of a genome by sequencing and assembling many short segments of genomic DNA

Question 4

Sequence contigs

Answer 4

A group of overlapping cloned segments

Question 5

DNA sequencing library

Answer 5

A set of DNA fragments that will be used for sequencing

Question 6

Transcriptome

Answer 6

The sequence and expression patterns of all RNA transcripts (which kinds, where in tissues, when, how much)

Question 7

Proteome

Answer 7

The sequence and expression patterns of all proteins (where, when, how much)

Question 8

Interactome

Answer 8

The complete set of physical interactions between proteins and DNA segments, between proteins and RNA segments, and between proteins.

Question 9

Two-hybrid test

Answer 9

A method for detecting protein-protein interactions, typically performed in yeast

Question 10

Chromatin immunoprecipitation (ChIP)

Answer 10

The use of antibodies to isolate specific regions of chromatin and to identify the regions of DNA to which regulatory proteins are bound

Question 11

ChIP-seq

Answer 11

A variation of the ChIP procedure in which all of the DNA binding sites of a regulatory protein in a genome are identified by sequencing

Question 12

RNA interference (RNAi)

Answer 12

A system in some eukaryotes to control the expression of genes through the action of siRNAs.