Final - Ch 14 achieve

Question 1

Whole genome shotgun sequencing in order

Answer 1

• isolate genomic DNA
• break DNA into large fragments that are approximately 150,000 base pairs in length
• insert DNA back into BACs
• break DNA into fragments that are approximately approximately 1000 base pairs in length
• insert DNA into plasmids
• sequence DNA
• computationally assemble sequences from each BAC
• computationally assemble the genome from overlapping BACs

Question 2

What is a contig?

Answer 2

a continuous DNA sequence constructed from overlapping DNA fragments