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Microbiology Lab > Final > Flashcards

Final Flashcards

1
Q

Algae

A

single celled, chlorophyll

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2
Q

amylase

A

enzyme that breaks down starch

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3
Q

antiseptic

A

stuff that kills microbes on living tissue

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4
Q

aseptic techniques

A

washing hands and sterilizing of instruments, minimizing contamination

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5
Q

budding

A

form of reproduction, yeasts

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6
Q

casein

A

protein in milk

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7
Q

catalase

A

enzyme that breaks down hydrogen peroxide

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8
Q

CFU

A

colony forming units

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9
Q

Three factors that will cause the concentration of bacteria in milk to be high

A

a diseased utter, unsanitary handling/contamination, storage temperature

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10
Q

Showed that the childbed fever in Vienna hospital decreased when doctors and medical students washed their hands in lime water

A

Ignaz Semmelweis

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11
Q

British surgeon who promoted the soaking of instruments and the hand washing of hands with carbonic acid to prevent post-surgical infection

A

Joseph lister

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12
Q

contrast

A

stains of dyes, must have to be seen under a microscope

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13
Q

curds

A

milk protein casein

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14
Q

cysts

A

endospore for protists

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15
Q

detritus

A

organic debris

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16
Q

dilution blank

A

blank of water

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17
Q

dimorphic

A

exist as yeast or mold

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18
Q

disinfectant

A

kill or stop the growth of pathogens on inanimate objects

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19
Q

endospore

A

spore when conditions get hard

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20
Q

epidemiology

A

study of transmittable diseases

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21
Q

eukaryotic

A

has membrane bound nucleus

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22
Q

heterotrophic

A

has to get food from outside source

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23
Q

hypha

A

branch or filament

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24
Q

index case

A

first case in a epidemiological study

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25
Q

magnification

A

magnifying something

26
Q

molds

A

grows in the form of hypha

27
Q

mordant

28
Q

whey

A

liquid that separates from the curds

29
Q

Two types of antimicrobial drugs

A

synthetic drugs and antibiotics (produced by organisms themselves)

30
Q

mycelium

A

vegetative part of fungus

31
Q

nosocomal infection

A

infection contracted in hospital

32
Q

parfocal

A

changed length of magnification

33
Q

prokaryotic

A

does not have bound nucleus

34
Q

protease

A

enzyme that breaks down protein

35
Q

protozoa

A

single celled eukaryote

36
Q

dilution factor

A

1/dilution

37
Q

pure culture

A

colonies that are all the same

38
Q

Protein hydrolysis

A

skim milk agar, casein is a protein-proteases digests protein. If bacteria produce this enzyme then the white casein will be digested on the plate

39
Q

resolution

A

the amount of detail able to be obtained

40
Q

three types of protists

A

algae, protozoa, fungi-like protozoa

-slime molds, amoeba, algae

41
Q

spectrum of activity

A

whether an antibiotic is broad or narrow spectrum

42
Q

characteristics of protozoa

A

single celled, mobile, heterotrophic, no cell well, eukaryotic

43
Q

Basic dye

A

contain ions that are positively charged and will be attracted to and color bacterial cells that tend to have negative charges

44
Q

Acidic dyes

A

ions that are negatively charged and are repelled by bacterial cells, stains background

45
Q

working distance

A

distance of object to lens

46
Q

yeasts

A

single cell fungus

47
Q

simple stain

A

basic dye, methylene blue, safranin, crystal violet

48
Q

differential stain

A

gram stain,

-stain with primary stain then counterstain

49
Q

Staining gram stains

A 
1-clean slide
2-six bacteria on slide
3-primary stain of crystal violet
4-mordant of iodine (crystalize)
5-decolorized with alcohol 
6-counterstain of safranin
50
Q

special stain

A

endospore, flagella

51
Q

special stain

A

endospore, flagella

52
Q

three types of protists

A

algae, protozoa, fungi-like protozoa

  • slime molds, amoeba, algae
  • heterotrophs, autotrophs, saprotrophs ,
53
Q

scanner

54
Q

low power

A

yellow, 10x*10x=100x

55
Q

high power

A

blue, 40x*10x=400x

56
Q

oil immersion

A

black and white, 100x*10x=1000x

57
Q

three domains

A

bacteria, archaea, eukaryota

58
Q

four kingdoms

A

animals, plants, protists, fungi

59
Q

characteristics of protozoa

A

pseudopodia, flagella, cilia, parasite

60
Q

four kingdoms

A

animals, plants, protists, fungi

61
Q

three things needed to grow bacteria

A

nutrients, avoiding contamination, isolate different species

62
Q

agar

A

easily rehydrated, a polymer of galactose, can be digested

Microbiology Lab (9 decks)

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