Final Flashcards
PGF2a
Must be cyclin and sensitive
5d-18d ➡️ regress CL ➡️✳️heat
Lutalyse, estrumate, estroplan, prostamate
P4
Pro-gestation: prep uterus
⛔️ov
Use implant/feed: removal ➡️✳️heat
Two shot method
- GnRH➡️ sync new dev fol wave
- PGF2a➡️kill CL
- GnRH ➡️ LH surge
30h ov & AI
7d cosync + CIDR
- GnRH + CIDR for 7d
- Remove CIDR + PG hit
66h GnRH + AI
Maternal recog
Hu: uterus➡️ hCG
Pig: embryo➡️E2:⛔️PGF2a
Ewe: embryo➡️IFN-T: ⛔️E2-R ⬇️OTr
Eq: unk small peptide
Placental attach
Mare + pig: diffue
Ru: cotyledonary
Hu: discoid
Cat+dog: zonary
Ejac➡️1st cleavage div
ICUOCBZF
BRBBR
Mucous Retrograde & neutrophils Cervical crypts Uterus: trap in ut gl; ❇️capi Uterotubal jct Oviduct: complete capacitation Isthmus❇️hyperactivity
Sperm capacitation
Removal of seminal fl + cholesterol protein coat ➡️ release binding from oviduct epi
Isthmus completes capi➡️✳️hyperactive sperm
Cumulus expansion
LH surge➡️❇️cum expansion
Change gap jct bw cells
Hyaluridonase-hyaluronic acid➡️ breaks down gap jct ➡️ penetration & fertilization
Sperm attach ZP
ZP3 binds Zona Binding Region➡️ ❇️acrosomal rxn ➡️ fuse PM + OAM
➡️Leak enz to break down ZP + enter perivit space
Sperm ova fuse
IZUMO on eq seg of sperm binds JUNO on ova PM➡️✳️fuse➡️ zona rxn + viti block ➡️ rel cortical gran + remove JUNO
- Complete meiosis I➡️2nd PB
- Cortical granules
Engulf➡️ decondense dna ➡️ pronuclei ➡️ duplicate & divide
⛔️polyspermy
Req fuse eq seg + PM
Zona rxn: slow➡️harden ZP +⬇️JUNO
Vit block: fast➡️ Ca2+ spikes:✳️cortical gran rel
Fl ➡️ blastocyst
Compaction: Morula forms tight jct
NaK pump on basal mem: Na in
H2O follows in & sep ICM with gap jct from trophoblast tight jct
Hatching
- Growth & fl in
- Trophoblast Enz production
- Blastocyst expand & collapse
Gestation days
Cattle: 285 Sheep: 149 Pig: 114 Mare: 340 Rat: 21 Dog: 65