FINAL 01 - Chromatography

Question 1

Is the ability to separate molecules using various partitioning characteristics of a molecule

Answer 1

Chromatography

Question 2

Chromatography was first applied by __________ for the separation of plant pigments

Answer 2

Mikhail Semyonovich Tsvet

Question 3

Greek word which means ‘color’

Answer 3

Chromatos

Question 4

Greek word which means ‘to write’

Answer 4

Graphein

Question 5

2 major components of chromatographic separation

Answer 5

Mobile phase, Stationary phase

Question 6

Flows through the column; carries analyte

Answer 6

Mobile phase

Question 7

Stays in place, does not move

Answer 7

Stationary phase

Question 8

Instrument employed for a chromatography

Answer 8

Chromatograph

Question 9

Phase that stays in place inside the column

Answer 9

Stationary phase

Question 10

The solvent moving through the column; either a liquid in LC or gas in GC

Answer 10

Mobile phase

Question 11

Fluid entering the column

Answer 11

Eluent

Question 12

Fluid exiting the column

Answer 12

Eluate

Question 13

Is the process of passing the mobile phase through the column

Answer 13

Elution

Question 14

Graph showing detector response as a function of time

Answer 14

Chromatogram

Question 15

Defines how much mobile phase passes per minute (mL/min)

Answer 15

Flow rate

Question 16

Distance passed by mobile phase per 1 min in the column (cm/min)

Answer 16

Linear velocity

Question 17

Red pigment in leaf

Answer 17

Anthocyanins

Question 18

Orange pigment in leaf

Answer 18

Carotenoids

Question 19

Yellow pigment in leaf

Answer 19

Xanthophyll

Question 20

Green pigment in leaf

Answer 20

Chlorophyll

Question 21

Rf value of beta-carotene

Answer 21

0.99

Question 22

Rf value of chlorophyll A

Answer 22

0.30

Question 23

Rf value of chlorophyll B

Answer 23

0.13

Question 24

Rf value of violaxanthin

Answer 24

0.40

Question 25

Rf value of lutein

Answer 25

0.68

Question 26

Relationship of Rf and polarity

Answer 26

Inversely proportional (higher Rf, lower polarity and vice versa)

Question 27

Relationship of Rf and solubility

Answer 27

Directly proportional (higher Rf, higher solubility and vice versa)