Fatty Acid Oxidation

Question 0

hormone sensitive lipase is regulated by

Answer 0

PKA (activation step regulated by glucagon/epinephrine)

Question 1

Hormone sensitive Lipase degrades

Answer 1

diglycerides to monoglycerides

Question 2

2 forms of HS Lipase

Answer 2

long-cholesterol metabolism for steroid production

short-in adipocytes, hydrolyzes triglycerides to free FAs

Question 3

Step 1 of FA metab

Answer 3

FAs activated in cytoplasm

enz: fatty acyl CoA ligase

Question 4

Step 2 of FA metabolism

Answer 4

transport FA to mitochondria

Question 5

Step 3 of FA metab

Answer 5

beta oxidation in mitochondria

Question 6

1st step in FA oxidation

Answer 6

FA mobilization

Question 7

Rate Limiting step of beta oxidation

Answer 7

carnitine + acyl CoA –> acyl carnitine

enz: Carnitine palmitoyl transferase I

Question 8

CPT I inhibited by

Answer 8

malonyl CoA (produced in FA synth)

Question 9

beta oxidation feeds into

Answer 9

electron transport chain

Question 10

each round of beta oxidation produces

Answer 10

1 acetyl CoA (12 ATP), 1 FADH2(2 ATP), and 1 NADH(3 ATP) = 17ATP

Question 11

enoyl-CoA isomerase

Answer 11

turns cis double bond into trans DB(which can be cleaved)

Question 12

alpha oxidation

Answer 12

occurs at alpha carbon; 1 C is removed from carboxyl end of FA chain and released as CO2
-required with presence of beta-branched methyl group (as with phytanic acid)

Question 13

Role of peroxisomes in beta ox of FAs

Answer 13

occurs when FAs are too long to be oxidized in the mito
not directly coupled to ATP production
electrons donated to O2 to generate H2O2 and heat

Question 14

FA synthesis is stimulated by

Answer 14

insulin, glucose, citrate

Question 15

FA synthesis is inhibited by

Answer 15

glucagon, malonyl CoA

Question 16

FA oxidation is stimulated by

Answer 16

glucagon, Fatty acyl-CoA

Question 17

FA oxidation is inhibited by

Answer 17

insulin, glucose

Question 18

In Refsum disease, __________. Due to _____.

Answer 18

Phytanic acid can’t undergo alpha oxidation. Due to enzymatic deficiency (loss of PHYH gene)