Farvningsmetoder Flashcards

1
Q

HE-farvning

A

Hæmatoxylin farver basofile strukturer blå

Eosin farver acidofile substanser røde/lyserøde

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2
Q

Hæmatoxylin anvendes til:

A

Farvning af kernens DNA og cytoplasma (varieret farve)

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3
Q

Eosin anvendes til:

A

Cytoplasma

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4
Q

Van Giesons farvning

A

Farver:

  • Kollagen: rødt
  • Erythrocytter og cytoplasma: gult
  • Kerner: blå
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5
Q

Verhoeff farvning

A

Farver:

- Elastiske fibre og cellekerner: blå-sorte

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6
Q

Retikulin farvning

A

Farver:

  • Retikulinfibre: sort
  • Kollagene fibre: gul-brune
  • Erytrocytter: gule
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7
Q

Osmium-fixering anvendelse og farve

A

Anvendelse: fedtvæv
Farver:
- Fedtvæv (lipider): sort

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8
Q

Toluidin-blåt anvendelse og farve

A

Anvendelse: Kerne, cytoplasma, glykosaminoglykaner
Farver:
- Glykosaminoglykaner: røde/violette
- Basofile strukturer fx DNA, RNA: stærkt blå
- Cytoplasma: varierende farve

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9
Q

PAS farvning

A

Farver:

- Glykogen, glykoproteiner: røde

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10
Q

May-Grünwald Giemsa farvning

A

Farver:

  • Kerner: violette
  • Eosinofile granula: brun-orange / teglstensrødt
  • Basofile granula: rødviolet til mørkeblå
  • Neutrofile granula: svagt rød
  • Lymfocytters cytoplasma: gråblå
  • Thrombocytter: blå med blå granula
  • Erythrocytter: rosa
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