Factors Affecting Activity

Question 1

Effect of pH on activity

Answer 1

-low pH = lots of H+
-interfere with H + ionic bonds that hold the tertiary structure together
-eventually altering the shape of the active site

Question 2

Practical method into effect of pH on activity

Answer 2

-single drops of iodine in row on the tile
-label test tube with pH to be tested
-use syringe to place amylase in tube
-another test tube to add starch to amylase + buffer solution
-stopwatch
-after 10s pipette one drop onto first tile of iodine
-goes blue black
-stop until remains orange

Question 3

Effect of temperature on activity

Answer 3

-higher temp = move faster = more collisions
-collide with more energy

Question 4

Denaturation

Answer 4

-temp passes certain point
-increased KE + vibration - puts strain on weaker H + ionic bonds = start to break
-active site is permanently damaged - no substrate can bind

Question 5

What is the temperature coefficient?

Answer 5

-ratio between the rates of that reaction at 2 different temperatures
-reaction doubles for every 10 degree increase

Question 6

Enzyme concentration

Answer 6

-higher enzyme conc = more active sites available = more likely to form an enzyme substrate complex
-if amount of substrate is limited - certain point the conc will not increase
-becomes a limiting factor

Question 7

Substrate concentration

Answer 7

-greater concentration = higher rate of reaction - more likely for formation
-beyond certain point = rate of reaction no longer increases
-as enzyme conc becomes the limiting factor