FA Micro I Flashcards
What is the fxn and chemical composition of peptidoglycan
gives rigid support, protects against osmotic pressure, sugar backbone with cross linked peptide side chains
What is the fxn and chemical composition of cell wall/cell membrane in gram positive bacteria
major surface antigen, peptidoglycan for support, teichoic acid induces TNF and IL-1
What is the fxn and chemical composition of the outer membrane in gram negative bacteria
site of endotoxin (LPS), major surface antigen, lipid A induces TNF and IL-1, polysaccharide is the antigen
What is the fxn and chemical composition of the plasma membrance in bacteria
side of oxidative transport of enzymes, lipoprotein layer
What is the fxn and chemical composition of the bacterial ribosome
protein synthesis, 30S and 50S subunits
What is the fxn and chemical composition of bacterial periplasm
space between cytoplasmic membrane and peptidoglycan wall in gram neg bacteria contains many hydrolytic enzymes, including beta lactamases
What is the fxn and chemical composition of the bacterial capsule
protects against phagocytosis, polysaccharide (except for B. anthracis which contains D-glutamate)
What is the fxn and chemical composition of bacterial pilus/fimbria
mediate adherence of bacteria to cell surface;sex pilus forms attachment between 2 bacteria during conjugation, glycoprotein
What is the fxn and chemical composition of bacterial flagellum
motility, protein
What is the fxn and chemical composition of bacterial spore
provides resistance to dehydration, heat and chemicals; keratin like coat; dipicolinic acid
What is the fxn and chemical composition of plasmid
contains a variety genes for antibiotic resistance, enzymes and toxins; DNA
What is the fxn and chemical composition of the bacterial glycocalyx
mediates adherence to surfaces, especially foreign surfaces like indwelling catheters; polysaccharide
What cell wall structures are common to both gram pos and gram neg bacteria
flagellum, pilus, capsule, peptidoglycan, cytoplasmic membrane
What cell wall structures are found only in gram pos bacteria
teichoic acid
What cell wall structures are found only in gram neg bacteria
endotoxin/LPS, the periplasmic space (location of many beta lactamases)
what are the gram pos coccus (genus)
streptococcus, staphylococus
What are the gram neg coccus (genus)
Neisseria
What are the gram pos bacilli
clostridium, cornybacterium, bacillus, listeria, mycobacterium (acid fast)
Of the gram neg bacillus, which are not enterics
haemophilus, legionella, bordetella, francisella, brucella, pasteurella, bartonella, garderella
Of the gram neg bacillus, which ones are enterics
E. coli, shigella, salmonella, yersinia, klebsiella, proteus, enterobacter, serratia, vibrio, campylobacter, helicobacter, pseudomonas, bacteroides
Which gram pos bacteria have branching filamentous morphology
actinomyces and nocardia (weakly acid fast)
Which gram neg bacteria have pleomorphic morphology
rickettsiae, chlamydia (Giemsa)
Which gram neg bacteria are spiral
leptospria, borrelia, treponema
Which bacteria have no cell wall
mycoplasma - have sterols
What makes up the cell membrane of mycobacterium
mycolic acid, high lipid content
Which bacteria do not gram stain well because they are intracellular
rickettsia, legionella, chlamydia (lacks muramic acid in cell wall)
Which bacteris do not gram stain well because there is no cell wall
mycoplasma
Which bacteria don’t stain well because they are too thin to be visualized
treponema
What bacteria requires acid fast stain to visualize
mycobacterium
What stain shows legionella
silver stain
What is the technique to visualize treponema
darkfield microscopy and fluoresecent
What organisms do Giemsa stain pick up
borrelia, plasmodium, tryapanosomes, chlamydia
What organisms stain with PAS
used to diagnose Whipple’s disease - tropheryma whippelii
What organisms stain with Ziehl-Neelson
acid fast organisms
What organisms stain with india ink
cryptococcus neoformans
What organisms stain with silver stain
fungi (pneumocystis), legionella
What does PAS actually stain for
glycogen, mucopolysaccharides
what bug grows on chocolate agar with factors V (NAD+) and X (hematin)
h. flu
What bug grows on thayer-martin (or VPN) media - what does VPN stand for
N. gonorrhea - vancomycin (inhibits gram pos) polymyxin (inhibits gram neg), nystatin (inhibits fungi)
What bug grows on bordet-genou (potato) agar
bordetella pertussis
What bug grows on tellurite plate, lofflers media
C. diptheriae
What bug grows on lowenstein jensen agar
M. tuberculosis
What bug grows on eaton’s agar
m. pneumoniae
what bug grows pink colonies on MacConkey’s agar
lactose fermenting enterics
what bug grows blue black colonies on eosin-methylene blue agar with metallic sheen
E. Coli
what bug grows on charcoal yeast extract agar buffered with cysteine
legionella
What bug grows on sabouraud’s agar
fungi
What bugs are obligate aerobes
Nocardia, pseudomonas, m. tuberculosis, bacillus - nagging pests must breathe
In what clinical scenarios do you see Pseudomonas
burn wounds, nosocomial pneumonia, pneumonias in cystic fibrosis
Where does reactivation TB usually go, and what can precipitate reactivation
apices of lung, immune system compromise, or anti-TNF alpha use
What are the obligate anaerobes and what do they lack (and hence suscpetible to)
clostridium, bacteroides, actinomyces, lack catalse and superoxide dismutase and susceptible to oxidative damage
What are characteristics of obligate anaerobes
foul smelling (short chain fatty acids), difficult to culture, produce gas in tissues (CO2, H2)
Which Abx are ineffective against obligate anaerobes and why - where are anaerobes nl found
aminoglycosides, require O2 to enter bacterial cell - nl found in GI tract
What bugs are obligate intracellulars and why
rickettsia and chlamydia - can’t make out ATP
What bugs are the facultative intracellular
salmonella, neisseria, brucella, mycobacterium, listeria, francisella, legionella
What does the quellung reaction test for and what does a positive result indicate
encapsulated or not - positive if encapsulated bug is present; capsule swells when specific anticapsular antisera are added
What organisms are encapsulated
strep pneumo, klebesiella, H flu type b, N. meningititides, salmonella, group B strep
How do bacterial capsules function
antiphagocytic virulence factor
What is a capsule conjugated with a protein
antigen in vaccines
Why is the polysaccharide capsule conjugated to a protein
promote T cell activation and subsequent class switching - alone only IgM antibodies would be produced
What is in pneumovax
H. flu type B, meningococcal vaccines
What bugs are urease pos
proteus, klebsiella, H. pylori, ureaplasma - particular kinds have urease
What bugs produce yellow sulfur granules composed of a mass of filaments and formed in pus
actinomyces isreallii
What bug produces a yellow pigment
S. aureus
What bug produces a blue-green pigment
pseudemonas aeruginosa
What bug produces a red pigment
serratia
What virulence factor binds Fc region of Ig, prevents opsonization and phagocytosis and what bug has it
Protein A - S. aureus
What bugs can colonize the respiratory mucosa and why
S. pneumo, H. flu type b, Neisseira- SHiN - IgA protease cleaves IgA
What does M protein do, who has it
prevents phagocytosis - group A strep
This substance binds directly to MHC II and T cell receptor simultaneously, activating large numbers of T cells to stimulate release of IFN gamma and IL-2
superantigen
What is toxic shock syndrome, what bug secretes what substance to cause it
fever, rash, shock - S aureas, TSST-1
What toxins does S. aureus secrete
enterotoxins, TSST-1, exfoliatin which causes scalded skin syndrome
What bug secretes scarlet fever erythrogenic toxin and what does it cause
S. pyogenes, toxic shock-like syndrome
What do ADP-ribosylating A-B toxins do
interfere with host cell function, binding component binds to a receptor on surface of host cell enabling endocytosis - active portion attaches an ADP-ribosyl to a shost cell protein altering protein function
What kind of exotoxin does corneybacterium have and what does it do
ADP-R AB toxin - inactivates EF-2; causes pharyngitis and pseudomembrane in the throat (similar to pseudomonas exotoxin A)
What kind of exotoxin does V. cholerae have and what does it do
ADP ribosylation of G protein stimulates adenylyl cyclase, inc Cl- secretion into gut and dec Na absorption, H20 into gut lumen, voluminous rice water diarrhea
What kind of exotoxin does E. coli have and what does it work
ADP-R AB toxin: heat labile, stimulates adenylate cylcase, heat stable toxin stimulates guanylate cyclase - both cause watery diarrhea
What kind of exotoxin does bordetella pertussis have and what does it do
ADP-R AB toxin: inc cAMP by inhibiting Galpha1, causes whooping cough, inhibits chemokine receptor, causing lymphocytosis
What toxin does clostridium perfringens have and what does it do
alpha toxin, a lecithinase that acta s a phospholipase to cleave cell membranes and causes a gas gangrene