F9 Flashcards

1
Q

A common laboratory method of cultivating anaerobic microorganisms is
Gas pack system
Brewer jar system
Pogallic acid over the cotton
None of these

A

Gas pack system

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2
Q

An inoculated thioglycollate medium culture tube is clear throughout the tube except for dense growth at the bottom of the tube. What is your conclusion?
The organisms are obligate anaerobes.
The organisms are facultative anaerobes.
The organisms are aerotolerant.
The organisms are obligate aerobes

A

The organisms are obligate anaerobes.

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3
Q

Which of the following microorganisms form best at 30-37 degrees Celsius?
Psychophiles
Mesophiles
Thermophiles
Hyperthermophiles

A

Mesophiles

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4
Q

Which temperature range do bacteria grow best at?
35-37 degrees Celsius
50-60 degrees Celsius
20-40 degrees Celsius
15-20 degrees Celsius

A

35-37 degrees Celsius

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5
Q

The edge of the colonies is described as all the following EXCEPT:
Smooth
Wavy
Filamentous
Irregular

A

Smooth

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6
Q

All of the following are sediments found in liquid broth, EXCEPT?
I. Granular
II. Saccate
III. Flocculent
IV. Crateriform
V. Flaky
1 and 5
2 and 3
2 and 4
3 and 4

A

2 and 4

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7
Q

Which of the following are the proper conditions for strict anaerobes culturing?
I. Using specialized oxygen-free transport media
II. Processing the specimen in oxygen-free atmosphere
III. Using anaerobic jar for culturing
IV. Keeping seeds in the 370C incubator
if only 1, 2 and 3 are correct
if only 1 and 3 are correct
if only 2 and 4 are correct
if only 4 is correct
if all are correct

A

if only 1, 2 and 3 are correct

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8
Q

A technician is reading stool culture plates. She is looking for enteric pathogens on the MacConkey (MAC)plate. What do they look like?
I. Clear, colorless colonies
II. Large, bright pink colonies
III. Small, mucoid, green colonies
IV. Small, orange colonies
If only I is correct
If I and II is correct
If I, II and III is correct
All is correct
None is correct

A

If I and II is correct

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9
Q

On Mac Conkey’s medium, E. coli forms what colonies?
Colorless colonies
Greenish pigmentation
Pink-colored colonies
Medusa’s head appearance

A

Pink-colored colonies

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10
Q

Generally,microbiologists observe the colonial morphology of organisms on primary culture after how many hours of incubation?
18 to 24 hours
6 to 12 hours
24 to 48 hours
72 hours

A

18 to 24 hours

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11
Q

What is the pH required for the growth of bacteria?
6.8 7.2
5.6-8.2
.3.0-6.0
8.0-14.0

A

6.8 7.2

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12
Q

How much Oxygen is needed by Capnophiles?
5-10%
15%
0%
21%

A

15%

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13
Q

Which of the following best describes the odor of P. aeruginosa?
Old socks
Putrid
Grapelike
Freshly plowed field

A

Grapelike

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14
Q

Which of the following bacterium exhibits alpha hemolytic activity?
Streptococcus pyogenes
Staphylococcus aureus
Enterococcus faecalis
Staphylococcus pneumoniae

A

Staphylococcus pneumoniae

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15
Q

An inoculated thioglycolate medium culture tube shows dense growth at the surface and turbidity throughout the rest of the tube. What is your conclusion?
The organisms die in the presence of oxygen.
The organisms should be grown in an anaerobic chamber..
The organisms are obligate aerobes.
The organisms are facultative anaerobes.

A

The organisms die in the presence of oxygen.

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16
Q

Why do the instructions for the growth of Neisseria gonorrheae recommend a CO2-enriched atmosphere?
It is a capnophile.
It fixes CO2 through photosynthesis.
It uses CO2 as a final electron acceptor in respiration..
It is an obligate anaerobe.

A

It is a capnophile.

17
Q

The Gaspak system is suitable for which of the following?
Aerobic bacteria
Anaerobic bacteria
Facultatively anaerobic bacteria
Microaerophilic bacteria

A

Facultatively anaerobic bacteria

18
Q

The microorganisms that grow at high salinity are called?
Osmophiles
Halophiles
Both a and b
None of these

A

Both a and b

19
Q

Examine the statements below and determine which is correct.
I. Beta hemolysis is characterized by clear zones II. Alpha hemolysis is characterized by greenish zones
First statement is TRUE, Second statement is FALSE
First statement is FALSE, Second statement is TRUE
Both statements are TRUE
Both statements are FALSE

A

Both statements are TRUE