F8 Flashcards
Which of the following types of culture is prepared for parasites associated with many known organisms?
Xenic Culture
Polyxenic Culture
Monoxenic Culture
Axenic Culture
Polyxenic Culture
Which of the following is NOT a method of inoculation?
Streak Plate
Pour Plate
Spread Plate
None of the choices
None of the choices
When sterilizing inoculating loops and needles, which should NOT be considered?
sterilize before and after use
do not blow heated loops
wrapped loops should be opened on both ends
remove the peel of wrapped loops backward
wrapped loops should be opened on both ends
Which of the following should be considered for an aseptic technique using Petri plates?
The cover should not be placed on the bench top
Do not breathe on open plates
Remove the cover only when needed
All of the choices are correct
All of the choices are correct
What is the temperature of the melted agar where the diluted sample is mixed in the pour plate method?
30 degrees Celsius
45 degrees Celsius
50 degrees Celsius
75 degrees Celsius
50 degrees Celsius
Which of the following methods, the sample is diluted in distilled water several times, then the most diluted sample will be mixed with melter agar at 50 degrees Celsius, then poured into the Petri plates?
Streak Plate (Quantitative)
Streak Plate (Semi-Quantitative)
Pour Plate
Pour Plate
Which of the following is used for tissues from burn victims and urine culture?
Streak Plate (Semi-Quantitiave)
Streak Plate (Quantitative)
Pour Plate
Spread Plate
Streak Plate (Quantitative)
Among these inoculation methods, which is done in a tube and the sample is stabbed into the agar approximately from the surface of the agar.
Broth
Slant
Butt
Butt-Deep
Butt
Which of the following should NOT be done when cleaning the space inside the hood before use?
Spray the working surface with 70% alcohol solution
Any objects introduced inside shall be disinfected, for sterile objects, it’s not necessary
Keep hands within the cleaned area of the hod
Spray the exterior of the gloves with disinfectant
Any objects introduced inside shall be disinfected, for sterile objects, it’s not necessary
Which of the following should be done when preparing a butt tube media?
I. A sample is stabbed into the agar
II. The inoculating loop should be used
III. The sample should be approximately % from the surface of the agar
IV. The sample should be approximately ½ from the surface of the agar
I, II are correct
I, III are correct
I, II, III are correct
I, II, IV are correct
I, III are correct
Arrange the following steps in chronological order. The steps may be repeated many times. Choose the BEST answer.
I. Pull out the loop
II. Flame the mouth of tube
III. Sterilize the loop
IV. Insert the loop into the tube for an inoculum
V. Mix the loop with the media by stirring slowly
VI.Re-cap and incubate
III, II, IV, V, I, II, III, VI
III, IV, II, I, V, III, II, VI
III, II, III, I, V, IV, II, VI
III, I, II, III, II, IV, V, VI
III, II, IV, V, I, II, III, VI
Which of the following is done in preparing a deep-slant method?
A sample is streaked first, then stabbed using a sterile inoculating needle
A sample is stabbed for 3 from the surface of the agar and streaked in a zigzag pattern
A sample is stabbed first in the deep part, then streaked using a sterile inoculating needle
None of these are correct
A sample is stabbed first in the deep part, then streaked using a sterile inoculating needle
Which of the following types of culture is prepared for parasites associated with other unknown microbial associates?
Xenic Culture
Polyxenic Culture
Monoxenic Culture
Axenic Culture
Xenic Culture
Which of the following types of culture is prepared for parasites associated with other unknown microbial associates?
Xenic Culture
Polyxenic Culture
Monoxenic Culture
Axenic Culture
Xenic Culture
How many disposable inoculating loops shall be prepared for the Semi-Quantitative Streak Plate?
1
2
3
4
4
Which of the following is the purpose of flaming?
to sterilize
create air convection to prevent the entry of dust particles
kill bacteria
immobilize bacteria
kill bacteria
Which of the following is the purpose of a HEPA Filter?
captures a minimum of 99.97% of dust, pollens, and molds
captures any airborne particles with a size of <0.3 um
captures a minimum of 95% of dust, pollens, molds
captures any airborne particles with a size of <0.2 um
captures a minimum of 99.97% of dust, pollens, and molds
Which of the following is matched correctly?
Pure Culture - colonies originate from different species
Monoxenic Culture - Culture of parasites grown with other unknown microbial associates
Axenic Culture- Pure parasitic culture without any metabolizing cell
Xenic Culture - Culture of parasites grown in association with a single known microorganism
Axenic Culture- Pure parasitic culture without any metabolizing cell
Which of the following is TRUE about the different types of Inoculating Techniques?
I. Semiquantitative Streak Plate method uses a clock-streak technique
II. The quantitative Streak Plate only works if the metal inoculation loop is sterilized for each quadrant
III. 20 degrees angle for slant is used for anaerobic culture of microorganisms
IV. A sample is streaked in a zig-zag pattern using a sterile inoculation needle for slant tube technique
I, III, IV
I, IV
II, III, IV,
I, II, III, IV
I, III, IV
Which is INCORRECT when scoring in the Semiquantitative Streak Plate Method?
1+- 1st Quadrant should have less than 10 visible colonies
2+- 1st Quadrant should have less than 10 visible colonies
3+-3rd Quadrant should have greater than 10 visible colonies
4+-3rd 3rd Quadrant should have greater than 10 visible colonies
3+-3rd Quadrant should have greater than 10 visible colonies
Which of the following is TRUE when cleaning and disinfecting the space inside the hood after use?
The surfaces should be cleaned with 10% Hypochlorite solution
UV light should be tuned on while cleaning the surfaces
UV light helps keep down levels of potential contaminants.
All of the choices are correct
UV light helps keep down levels of potential contaminants.
Which of the following is TRUE when cleaning and disinfecting the space inside the hood after use?
The surfaces should be cleaned with 10% Hypochlorite solution
UV light should be tuned on while cleaning the surfaces
UV light helps keep down levels of potential contaminants.
All of the choices are correct
UV light helps keep down levels of potential contaminants.
