F7 Flashcards
An ingredient in a culture medium that resists change in pH of the medium
yeast
fermentable compounds
buffers
electrolyte
buffers
i. In Slant-Butt, the slant serves as the aerobic area and the butt serves as the anaerobic area.
ii. Slant-Butt contains 2 layers for the purpose of examining both aerobic and anaerobic organisms
1st statement is true. 2nd statement is false
1st statement is false, 2nd statement is true
both statements are true
both statements are false
both statements are true
viruses are best cultured in?
synthetic
non-synthetic
living
natural
living
A patient is suspected to be positive for gonorrhea, what medium can be used that is almost exclusive for the culture of the causative agent of this type of condition?
New York City Agar
Thayer Martin
Thioglycollate
Todd Hewitt broth
New York City Agar
Infusions or extracts of this material are considered the most component of broth medium
sheep liver
beef
pork
chicken
beef
The attending physician of WW-2 requested a culture of patient # 3’s specimen with the assumption of the presence of either Stretococci or Enterococci. What medium is best used in this situation?
Alkaline Peptone Water
Acetate Agar
Bile Esculin Agar
A7 and A8 Agar
Bile Esculin Agar
Differential media development was caused by a resistance of bacteria to an antibiotic. This is true in:
S. aureus being resistant to Methotrexate
Salmonella being resistant to Penicillin
S. aureus being resistant to Vancomycin
Mycobacterium being resistant to Rifampicin
S. aureus being resistant to Vancomycin
i. Enrichment medium can either be in broth or agar state; ii. Supportive media is not recommended for non-fastidious organism growth
1st statement is true; 2nd statement is false
1st statement is false; 2nd statement is true
both statements are true
both statements are false
1st statement is true; 2nd statement is false
Which state of culture media contains solid and liquid phases?
semi-solid
biphasic
slant
butt-slant
biphasic
Which tubed medium is best for growing or storing both aerobic and anaerobic bacteria?
Broth
Slant
Butt
Butt-Slant
Slant
Required for a definitive diagnosis
microscopic examination
accurate identification and characterization of bacteria
isolation and identification of viable pathogenic organisms on culture media
biochemical test
accurate identification and characterization of bacteria
Nitrogen requirement in the Culture medium can be found in what ingredient?
yeast extract
meat extract
peptone
electrolyte
peptone
What is the best course of action to prevent oxygen from entering the tube in a butt-tubed medium?
Seal with parafilm
drop mineral oil on top of agar
cork the tube
none of the selections
drop mineral oil on top of agar
Which type of culture media has known inorganic and/or organic compounds?
synthetic
non-synthetic
living
natural
synthetic
At what angle are tubed broth media usually positioned after preparation?
90 degrees
45 degrees
20 degrees
11 degrees
90 degrees
Still considered as the standard for diagnosis of infectious disease process
microscopic examination
accurate identification and characterization of bacteria
isolation and identification of viable pathogenic organisms on culture media
biochemical tests
isolation and identification of viable pathogenic organisms on culture media
A patient profile enters the laboratory with a preliminary assessment of Legionnaire’s disease. As the bacteriologist in charge, what media would you use?
Thioglycolate
Buffered-Charcoal Yeast Extract Agar
Sabouraud’s Dextrose Agar
Tryptic Soy Agar
Buffered-Charcoal Yeast Extract Agar
Peptone is the major ingredient of this type of medium
synthetic
non-synthetic
living
natural
non-synthetic
When choosing a culture medium, which is a characteristic of an ideal culture medium?
Cheap and reproducible
can grow a single bacteria satisfactorily
all selections apply
none of the selections apply
all selections apply
