F lec 19 Flashcards
(42 cards)
defined as a delay or arrest in the cell cycle progression in response to problems completing a specific step in the cell cycle or in response to other cellular problems
cell cycle checkpoint
cell cycle checkpoints may pause the cell cycle and promote ______________ before continuing - can also induce _____________ ____________ or _______________
repair, permanent arrest, apoptosis
cell cycle checkpoints are essential for protecting against _______________
cancer
a lot of genes involved in checkpoints are important _______________ ____________________ _____________
tumor suppressor genes
the _________ is recruited to unattached kinetochores
SAC (spindle assembly checkpoint)
the SAC is recruited to ________________ __________________
Unattached kinetochores
the ____________ component of the SAC is what gets recruited to the unattached kinetochore, being activated and then released
MAD2
once activated at the kinetochore, Mad2 binds and inhibits ______-_______
APC-Cdc20
Mad2 component of the SAC binds and ___________ APC-cdc20 - allows spindle to set up properly before anaphase is initiated
inhibits (keeps the APC inactive until the last of the improperly bound chromosomes is properly bound to kinetochore microtubules)
the _____ _________ ______________ _______________ is activated if DNA damage is detected in G1, leading to G1 arrest - this allows the cell cycle to pause while DNA is repaired - there is a similar checkpoint that operates in G2 and prevents entry into mitosis
G1 DNA damage checkpoint
checkpoint triggered by telomere shortening - cell replication depends on the enzyme telomerase repairing telomeres, but not all cells have telomerase (only embryos and stem cells) so then the telomeres get shorter and shorter and eventually they get too short to divide, leading to permanent cell cycle arrest
replicative senescence (G0)
replicative senescence checkpoints protect against chromosome _______________
fusion
2 kinases that are recruited to sites of DNA damage
ATR, ATM
kinase that is recruited to double strand DNA breaks
ATM
kinase that is recruited to exposed single stranded DNA and other DNA damage (including stalled replication fork since there is exposed ssDNA there as well)
ATR
DNA damage checkpoints:
chk1, chk2
2 functions of ATM and ATR when recruited to sites of DNA damage
helps recruit repair machinery, activates a cell cycle checkpoint
DNA damage checkpoints:
reversible cell cycle arrest
once bound to its cyclin partner, a CDK still needs activating phosphorylation from a ________
CAK
enzyme that phosphorylates CDK1 to inhibit its activity
Wee1 kinase
__________ ____________________ removes the inhibitory phosphate placed on CDK1 by Wee1 kinase
cdc25 phosphatase
_________ ______________ is necessary to activate CDK1 and CDK2 because it removes the inhibitory phosphate
cdc25 phosphatase
phosphorylation of cdc25 by chk1 and chk2 leads to its ___________________
degradation
phosphorylation of cdc25 by chks (which degrades it) and by cdks (which activates it) seems contradictory, but still can be so because the phosphorylations are occurring at ______ _______________ ____________ of the cdc25 protein
2 different sites