F: Continuous culture Flashcards
1
Q
Is a batch culture a closed system
A
yes
2
Q
What happens to bacteria in a closed system
A
grow in log phase for a few generations and then enter stationary phase
3
Q
What happens in an open system
A
environmental conditions are constant, bacterial growth can be maintained using a continuous culture system
4
Q
Describe chemostat
A
sterile medium is added to a culture vessel at same rate as medium containing bacteria is removed.
5
Q
How is the growth rate controlled
A
medium contains one nutrient in limiting quantities. eg the source of carbon or nitrogen
6
Q
How is the growth rate determined
A
by the rate the fresh medium is being added
7
Q
D=F/V
A
D=the dilution rate (per hour)
F=flow rate ml/hr
V=vessel volume ml
