eye piece graticule

Question 1

what is a eye piece graticule?

Answer 1

inside of light microscopes there is a scale on a glass disc which is called the eye piece graticule

Question 2

what is the eye piece graticule used for?

Answer 2

used to measure the size of objects you are viewing under the microscope
however, each time you change the objective lens, therefore also the magnification. you have to calibrate the eye piece to work out what the distance is between each division

Question 3

what is the method of calibration for the eye piece graticule?

Answer 3

1. line up the stage micrometre and the eye piece graticule whilst looking through the eye piece
2. count how many divisions on the eye piece graticule fit into one division on the micrometre scale
3. calculate the distance in micrometres of one division on the eye piece graticule