aseptic technique

Question 1

what is aseptic technique?

Answer 1

is a process used to achieve asepsis to prevent the transfer of potentially pathogenic micro-organisms to a site that may cause infection

Question 2

why do we need aseptic technique?

Answer 2

prevents spread of pathogens

Question 3

when is aseptic technique used?

Answer 3

• handling surgical equipment
• sterilisation of work surfaces
• reducing contamination from the environment
• prevent studied organisms being contaminated
• helping baby birth by vaginal deliver

Question 4

how do you maintain aseptic conditions in a lab?

Answer 4

• use of airflow cabinets
• using sterile loops when transferring cultures
• sterilising or disposing used equipment
• washing hands thoroughly

Question 5

what does sterile mean?

Answer 5

free from micro-organisms

Question 6

Labatory work that requires aseptic technique?

Answer 6

• cell and tissue culture
• prep of medical test kit
• pharmaceutical production of drugs and medicines
• microbiology and surgical procedures

Question 7

how do you streak a plate using aseptic technique?

Answer 7

1. sterilise the loop in the Bunsen burner until it turns red
2. let the loop cool and take an isolated colony from the agar plate
3. spread the isolated colony on the first quadrant of the petri dish
4. put the loop and pack away
5. flame the loop and pack away
6. incubate the plate for 24 hrs

Question 8

what is contaminated culture in biology?

Answer 8

a culture in which bacteria from a foreign source have infiltrated the growth medium

Question 9

what is plant tissue culture?

Answer 9

is a collection of techniques used to maintain or grow plant cells, tissues or organs under sterile conditions

Question 10

why is contamination bad?

Answer 10

• cause experiments to be repeated, resulting in delays

- costly wastage

Question 11

what is the cauliflower cloning method?

Answer 11

1. place forceps in sterilise solution called SDICN
2. clean the bench and wipe area
3. collect a cauliflower and place it on a petri, using a scalpel and cut it into a smaller part
4. put the cauliflower in a jar. every 2-3 mins, swirl the jar gently. repeat for 15 mins
5. strain the liquids and put the cauliflower into a agar growth medium
6. label the petri dish and incubate in a warm lab

Question 12

what are potential errors that can occur while streaking a plate?

Answer 12

• large amounts of inoculum used
• heavy streaking, agar is gouged
• drops of moisture on the plates surface
• loop not flames

Question 13

what is a growth medium?

Answer 13

is a solid, liquid or semi-solid designed to support the growth of a population of micro-organisms or cells. is often specific to the bacteria

Question 14

what are agar plates?

Answer 14

petri dish that contains a growth medium solidified with agar to culture micro-organisms