extreme biology Flashcards
give some environmental factors that plants need to be able to cope with
drought cold heat light salinity flooding nitrogen poverty wind loneliness cold
what do plants rely on light for
photosynthesis
what are the advantages and disadvantages of having large leaves in warm climates
advantages
- more area for light capture and photosynthesis
disadvantages
- exposed to a lot of heat
- photosynthesis releases a lot of heat which can be damaging
what doe ordinary sunlight at the surface of the earth contain and what is the effect of each of these
UV light - causes sunburn and is damaging to RNA/DNA
visible light - plants use the blue and red end of the visible light and reflect green light
infrared light - not used in biochemical pathways and is damaging in terms of heat
which part of the leaves are most affected by heat and how can heat damage be reduced
leaves get hotter away from their edges. by increasing the amount of edge a leaf has, the more heat loss radiation can be carried out
how does leaf size adapt to get more light
make the leaf bigger to get more sunlight
how does leaf size adapt to evade high temperature damage
make the leaf smaller to increase heat loss by radiation
how can leaf shape help to protect against over heating
we can change the orientation of leaves to avoid direct sunlight
so leaves also change shape i.e. curl up
apart from radiation, what are other methods of heat loss form leaves
transpiration of water helps plants keep cool do that their biochemistry isn’t wounded
in what conditions are small leaves favourable
extremes of dryness, heat, draught and cold
in what conditions are large leaves favourable
where there is sufficient water for cooling and it is warm enough to avoid excessive radiative cooling
large leaves maximise light harvesting
what adaptations have desert plants undergone
they are overexposed to heat so they have small leaves so that they can effectively radiatively cool because they don’t have access to enough water to cool by transpiration
what kind of plants do we get in hot treeless deserts
ephemerals, adaptors, resistors
describe the plant growth cycle in hot treeless deserts
in the dry season almost no plants grow but when it rains there is a massive bloom
what are ephemerals
they are desert annuals
they have a short life time (weeks/months) but seeds are long lived
energy form photosynthesis in leaves allows them to produce flowers. insects then pollinate them
the plant dies and the seeds are left in the soil/sand and when it rains more plants grow
what are the 4 main steps of ephemeral life cycle
growth
flowering
seed setting
death
are ephemerals draught resistant
not particularly
what is cryptobiosis
a metabolic state entered by organisms in response to adverse environmental conditions
what are poikilohydric plants and give an examples
they are resurrection plants craterostigma plantagineum
what is the response of resurrection pants in draught
during draught the plants dehydrate, shrivel and become photosynthetically inactive
it then regrows from dormant roots and shoots when watered
describe a dehydration protection response of resurrection plants
- accumulation of sucrose and trehalose to protect membranes and proteins from denaturation
- when it is dry it allows all of the tissues to shrink nut they don’t die.
- as the water content goes down it starts to produce more sucrose which is a good cryo-preservative as the sugar acts against drying
how do poikilohydric (resurrection) plant and ephemerals differ
ephemerals die and leave their seeds
resurrection plants don’t die
what are phreatophytes
deep rooted large perennial plants that obtain a significant portion of water from the phreatic zone
what is the phreatic zone
the zone of saturation
what are the 2 roost formed by the phreatophytes
deep tier roots that get water from the deep water table
shallow tier roots that capture rainfall
describe the leaves of phreatophytes
they are tiny in order to reduce water loss and aid radiative heat loss
are stomata open or closed during the day in phreatophytes
they are closed to prevent water loss s
give a example of a phreatophyte
creosote bush
mesquite plant
what is the oldest plant
the larrea tridentata found in the death valley is thought to be the oldest creosote bush ring estimated at 11700 y/o reaching up to 20m in diameter
it is a phreatophye
what are xerophytes
- plants that can survive very dry conditions for long periods of time and are found in arid climates
- they usually have small leaves or needles
- they have thickened leaves or stems for water storage and to act as a heat buffer
what is an arid climate
climate with low water availability
describe the succulents of xerophytes
succulents contain storage of water in very sugary cores and they are found in leaves and stems
give examples of xerophytes and note the type of succulent they have
aloe vera and crassula - leaf succulents
cactus - stem succulents
describe cacti in terms of their leaves and stems
- cactus spines are their leaves but they are not for photosynthesis, instead they are for protection
- the entire stem of the cactus is photosynthetic
what are cacti areola
an area on the plant where a tuft of white hairs come out
give reasons why cacti are very good at retaining water
- because they have very waxy cuticles and water succulents
- they have reduced SA to volume ratio
- stomata are strategically concentrated inside the pleats (shaded most of the time) to reduce desiccation when they are open so that sunlight doesn’t drive off too much water
- cacti apex are generally covered in hairs - the hairs protect it from light and heat damage - no photosynthesis occurs here
what conditions are high altitude cacti adapted to
dry, cold air and intense light
what does it mean that high altitude cacti are very pubescent and how is this of benefit
they are very hairy
- this results in light being scattered to prevent damage but it can still be let through for photosynthesis
- when stomata are open they lose less water du to the microenvironment created by the hair
- the hairs also prevent heat loss at night
- protection from herbivores and pathogens
high altitude cacti are long/short lived and slow/fast growing
long
slow
what kind of conditions are euphorbias adapted to
dry conditions
how do euphorbias protect themselves against other animals and how are they different to cacti
they oose whit latex to protect themselves against grazing by insects and other animals but cacti can’t doe this
cacti have areola but euphorbias don’t
what are lithops and what is there nickname
they are called stone plants because they look like stones
- their outer leaves shed in the winter and are replaced by new ones (they need to get rid of old leaves because they are light damaged)
what is a compass plant
a plant that reduces heat and water loss native to new mexico
- it reduces the heating of planar faces
- they change leaf orientation so that they can still photosynthesise but prevent light and heat damage (this orientation increases water efficiency)
- they have large leaves which are held vertically
- new leaves grow in random orientation but within 2-3 weeks it twists on its petiole to a vertical position
are most plants C3 or C4
C3
what are the differences between C3 and C4 metabolism
C4 metabolism is a photosynthetic pathway that adapted from the C3 pathway in response to extreme heat and drought. It evolved independently in several lineages of vascular plants. The C4 pathway works most effectively in hot and dry climates with limited CO2. It is called C4 because instead of forming a 3-carbon compound (as in the C3 pathway), it makes a 4-carbon compound (malate).
what are the advantages of C4 metabolism in plants
- In hot, dry environments C4 metabolism is more efficient than C3 because it minimizes photorespiration, a counterproductive pathway to photosynthesis where rubisco incorporates oxygen instead of carbon dioxide. C4 plants minimize photorespiration by separating carbon fixation and the calvin cycle in space or in time. In both pathways, carbon dioxide is initially incorporated into the plant by an enzyme called PEP carboxylase which has no affinity for oxygen (avoiding photorespiration). The benefits of reduced photorespiration exceed the energy cost of C4 pathways
- C4 plants also reduce water loss by transpiration because they can keep their stomata closed more than C3 plants. The plants need to open their stomata in order to take in carbon dioxide and release oxygen into the atmosphere. During this time, water is lost by transpiration which is enhanced in hot and dry climates. The C4 pathway is more efficient at incorporating carbon dioxide compared to the C3 pathway so the stomata can be kept closed more, thus avoiding water loss.
what are the two main type of C4 metabolism
There are two main types of C4 metabolism – one that separates carbon dioxide fixation and the calvin cycle with space (C4 pathway) and one that separates them with time (CAM). In the C4 pathway, plants open their stomata during the day and carbon fixation occurs in the mesophyll cells where carbon dioxide is incorporated by PEP carboxylase forming malate. Malate is then transported to the bundle sheath cells via plasmodesmata and the carbon dioxide is released and incorporated into the calvin cycle by rubisco. In CAM plants, stomata are opened at night. Carbon dioxide is fixed to oxaloacetate by PEP carboxylase (the same as in the C4 pathway), then converted to malate or another type of organic acid which is stored inside vacuoles until the next day. In daylight, the CAM plants do not open their stomata, but still photosynthesize because the organic acids are transported out of the vacuole and broken down to release carbon dioxide which enters the Calvin cycle.
what are the differences between the 2 types of C4 metabolism
In the C4 pathway, plants open their stomata during the day and carbon fixation occurs in the mesophyll cells where carbon dioxide is incorporated by PEP carboxylase forming malate. Malate is then transported to the bundle sheath cells via plasmodesmata and the carbon dioxide is released and incorporated into the calvin cycle by rubisco. In CAM plants, stomata are opened at night. Carbon dioxide is fixed to oxaloacetate by PEP carboxylase (the same as in the C4 pathway), then converted to malate or another type of organic acid which is stored inside vacuoles until the next day. In daylight, the CAM plants do not open their stomata, but still photosynthesize because the organic acids are transported out of the vacuole and broken down to release carbon dioxide which enters the Calvin cycle.
what percentage of plants are C4
3% but 5% of biomass and 23% carbon fixation
how much less water do C4 plants lose compared to C3
C4 plants lose 1/2 as much water per unit CO2 compared to C3 plants
what is the most abundant protein on the planet
rubisco
what does rubisco do
it fixes CO2 into sugars in plants nut it is very sensitive to oxidation by pure oxygen and it evolved when oxygen levels in the atmosphere were much lower so there was less selection pressure at the time to discriminate
the fact that rubisco can’t easily discriminate between oxygen and carbon dioxide lead to photorespiration
what does spatial separation mean in C4
physical separation of where CO2 is brought into the plant and where rubisco is active
CO2 brought into the mesophyll
rubisco active in bundle sheaths (active everywhere in C3 plants)
what increases the internal CO2 concentration
CO2 pumps - so that we generate an environment high in CO2 conc in the bundle sheathes
where is malate synthesised
in the mesophyll cells
hoe does malate get from the mesophyll cells to the bundle sheath cells
it diffuses via plasmodesmata
why can’t C3 plants grow in very hot areas
because with increasing temp rubisco incorporates more oxygen than CO2
what is marram grass
it is found on sand dunes and curls when dry and opens when wet = opening controlled by hinge cells
stomata are in the grooves to reduce water loss
what is the C4 time separation reaction called
CAM
what are the stomatal differences between the 2 C4 pathways
spatial separation - stomata closed at night and open during the day time separation (CAM) - stomata open at night and closed during day
what are the steps in CAM
CO2 is incorporated by PEP carboxylase and converted to oxaloacetate which is converted to malate which is converted to malic acid which is stored in the vacuoles during the day
during the day malic acid is moved to the cytoplasm and converted back to malate which is then pumped into the chloroplasts
CO2 is released and reacts with rubisco in the calvin cycle and pyruvate is released which goes back to the mesophyll cells
in what type of climates can slat be problematic for plants
marine and brackish waters and arid land areas
give examples of halophytic plants and their specialised salt organs and adaptive metabolisms
mangroves - slat glands - salt is secreted from glands
atriplex - slat bladders - produce gritty silver layer when excreting salt
mesembryanthemum crystallinum - ice plant - facultative CAM - shifts to CAM metabolism in dry or saline conditions
not all halophytes have obvious morphological features - provide an example
thellungia halophylia (salt tolerant) - close relative of Arabidopsis (salt sensitive)
what is the mechanism that allows thellungia halophylia to be salt tolerant
- ion channels in thellungia roots are less permeable for Na than in Arabidopsis
- limitation of Na uptake is the main strategy for avoiding sodium toxicity in thellungia
- thellungi host a potential set of genes that could be used to make our crop plants more salt tolerant
what are adaptations in plants to avoid anoxia from water flooding
pneumatophores - specialised aerial roots that enable access to O2 in water logged habitats
hypoxic roots - if roots are often flooded e.g. lotus, they produce aerenchyma tissue in their roots. cells within the roots die to produce channels through which air can diffuse allowing the system to respire even when flooded
what is an angiosperm
a plant that has flowers and produces seeds enclosed within a carpel(usually a fruit)
what is a gymnosperm
no flowers or enclosed seeds, seeds often cones e.g. conifers
describe seagrass (zostera marina)
it is an angiosperm with submerged male and female parts
what has seagrass lost genes for
it has lost all genes for stomata, UV protection (UV light only penetrates mm), IR light (cooled by water instead) and volatile terpenes (defence mechanism against predators)
what has seagrass gained genes for
encoding cell wall components for osmotic control to prevent saltwater driving water out of the plant
how can nitrogen poverty be evaded by carnivory
when plants eat insects it provides them with a nitrogen source -this is useful if the soil is nutrient poor
give examples of carnivorous plants and their mechanisms
venus fly trap - reversibly depolarises cells to open and close. its hairs need to be triggered twice before it will close
bladderwart - if bladders are triggered by touch they spring opne then suck in the insect and close a digest it
pitcher plants - produce vessels, sometimes with a lid to prevent fluid dilution. the vessel fluid digests insects that land in it
give an example of how parenthood can help with nitrogen poverty
coco de mer
lodoicea maldavica is a monodominant palm producing the largest seeds.
the plant invest heavily in reproduction
coco de mer has large leaves that gather up bird droppings, petioles etc from other plants and their own pollen
the rain washes it all down tubes that feed to the ground producing a nutrient rich environment for their offspring
this mechanism improves phosphorus and nitrogen supplies which are needed to sustain costly reproctive functions
what doe monodominant mean
when more than 60% of the tree canopy is comprised by a species
how are coco de mer and coconuts different
the seeds of coco de mer are not salt tolerant so they die if they fall in the sea unlike coconuts
describe the arctic tundra environment
cold deserts, biodiversity is very low
little rainfall
high densities of the same species
7 months of snow cover in winter - limited light capture
what kind of plants do we find in the arctic tundra
plants that have short growing seasons and life cycles in summer then go into senescence in winter
plants have high nutrient efficiency and live in the shallow soil above the permafrost (frozen rock/soil)
some plants are able to grow in rocks, give an example
lichens and mosses - able to tease nutrients out of the rocks
describe the Antarctica plants
must grow quickly and in extremely cold conditions
metabolic activity is maximised at low temperatures
there are only 2 native vascular plant species
what are the 2 native vascular plant species of the Antarctica
hairgrass
pearlwort
both of these grow in small clumps
how do lichens grow in the antarctica
they find spaces where the sun melts the snow
growth season is less than 120 days per year
growth rate is 0.01-1mm per year
long life
how do mosses grow in Antarctica
they form colonies that collect and retain water
their photosynthetic enzymes have maximal activity at 5C
photosynthesis quickly activates after frost
describe the plant growth in taiga
mainly coniferous but some angiosperms
plants can deal with cold and warm temperatures due ti the continental climate
there is low species diversity but large bands of single species
what is taiga
huge bands of forest
what is earth’s largest terrestrial biome
taiga
what are the dangers of cold to plants
- biological thermodynamic processes come to a halt
- changes in biomolecule conformation, stability and function
- perturbation of normal cellular processes
- reduced fluidity of membranes
- perturbation in the balance between production and neutralisation of ROS
- extracellular ice crystal formation depletes water in and around cells and crystals can puncture cell wall/memb and may rupture the inside of the plant
what do plants induce to protect themselves from the cold
cold tolerant mechanisms and components
describe the preparation of taiga conifer trees for winter
- they sense changes in day length/temperature which causes cold tolerant mechanisms to be triggered
- large central vacuole is replaced by lots of small vesicles to limit ice crystal formation
- starch granules disappear and other sugars accumulate
- thylakoid membranes separate and become disorganised
- changes in membrane lipid composition
- high concentrations of oligosaccharides to promote high viscosity of the cytoplasm of freeze dehydrated cells
- changes in proteins expressed
how do membranes change in response to cold
unsaturation, increased phosphatidylethanolamine and decreased phosphatidylcholine, increased phospholipids, reduced galactolipids
which proteins are upregulated in response to cold
dehydrins - prevent membrane-membrane interactions
antioxidant systems - protection against oxygen damage
heat shock proteins
pathogenesis related proteins
describe the leaves of deciduous trees
they generally have large light gathering leaves which are shed in winter to prevent the tree from being blown over
what is an abscission layer
it is a barrier across the petiole
it forms between the leaf petiole and the stem
- the leaf remains attached by the abscission layer as long as auxin is being produced i.e. the plant is growing
- when the plant prepares for winter auxin stops being produced
- the cells in the abscission layer start to separate and form a protective barrier to pathogens for when the leaf falls and prevent sap loss
when does the abscission layer form
in spring with new leaf growth
why are new buds sticky
because they coat themselves in sugar as a cryoprotective mechanism
what are response of leaves in winter that aren’t shed
- flat surfaces radiate away heat but don’t absorb much so they supercool
- to get around this, plants curl and drop their leaves in response to cold as this changes the airflow
- they also often have dense hair under the leaves which protect the stomata
give an example of a plant that drops and curls its leaves
rhododendron
describe the origin of conifers
- large continental mass called the Pangea formed which had a very dry centre because it was far from the sea
- this lead to the extinction of giant tree ferns
- conifers evolved and began colonising the continent changing its habitat
- conifers were likely the major food source of adult sauropods
give an example of a large conifer that was thought to feed sauropods
monkey puzzle tree
what is meant by conifers being evergreen
they are spring ready - have green leaves throughout the year
what conditions are conifers best adapted to
dry, cold and high altitude
how are conifers designed for snow
snow can land on them and their branches will bend and shed the snow if they get too loaded
angiosperms would probably break under the weight of snow
what can happen to photosynthesis in conifers in winter
some shut down photosynthesis and increase the conc of carotenoid pigments to protect against light damage
photosynthesis resumes after winter dormancy in response to daylight
the oldest bristlecone pine was accidently burnt describe the plants soil and root system
- they grow slowly in dolomite soils and the root system is composed of highly branched shallow roots while a few large branching roots provide support
- they are extremely draught tolerant with waxy needles, thick needle cuticles to aid water retention
- wood is very dense evading invasion by predators
crown drag is greatest in trees with narrow/broad leaves and the advantage is to be deciduous/coniferous
broad
deciduous
describe an oak tree (angiosperm) (deciduous)
dense heavy trunk
limited sway
broad root base
describe and pine tree (conifer) (gymnosperm)
lighter trunk
more sway
deep tap root
what is a buttress root
- most rainforest soil is nutrient poor and nutrients are available largely at the soil surface
- rainforest trees tend to have very shallow roots which can often be seen above the soil
how do trees/leaves reduce crown drag
- leaves twist, curl or fold to resist wind so they aren’t ripped off the tree or cause the tree to fall over
what can plant loneliness be caused by
distant colonisation
devastated land
widespread separation
give an example of a plant that grows on devastated land
rosebay willowherb - grows in ash - doesn’t require soil fungi - they have feathery seeds so that they can colonise land rapidly
give examples of plant loneliness - how do cacti combat loneliness
cacti are often spread out so need to try hard to find a pollinator
they often have improved advertising to attract pollinators
give an example of loneliness in a tropical forest
some species are not hermaphrodites and have male and female parts on different plants so have to transfer pollen
plant species often use a pollinating insect instead of relying on wind
they synchronise their flowering using the solar analemma
why can’t temperate tropical rainforest plants or polar plants rely on temperature or daylight
because the environment is very stable
they use the solar analemma instead
what is the solar analemma
- a consequence of the earth’s rotational axis being at an angle of 23.5 to the plane of its orbital and earth’s orbital is elliptical
- plant detect tiny changes in when dawn occurs
- the analemma curve is bigger in the south because we have an elliptical orbit and we are tipped slightly towards the sun during the southern hemisphere’s summer
give some examples of common classes of extremophiles
acidophile alkaliphile halophile hyperthermophile oligotroph osmophile piezophile psychrophile thermophile
what is deinococcus radiodurans
a radiation surviving bacterium
- DNA organised into tightly packed choroids
- meat treated with radiation thought to kill all live nut meat still spoiled and DR was isolated
- radio-resistance of DR was a side effect of dealing with long term desiccation
how does DR repair DNA
it can repair single and double stranded DNA
- upon damage it brings its DNA into a compartmental ring like structure where it s repaired
- nucleoids then fuse from the outside of the compartment with the damaged DNA
what are the basic requirements for early life
carbon and nitrogen - they generate energy through cycles like the calvin cycle, krebs cycles and nitrogen cycle
what are the 3 domains of life
bacteria, archaea, eukaryotes
what are the different atmosphere states in its evolution
anaerobic, single cells
anaerobic, photosynthesis, single cells
aerobic, eukaryotes
multicellular life, Cambrian explosion, stability
what are the effects of oxygenation of the atmosphere
- oxygenation of the atmosphere by photosynthetic pigments is thought to have caused precipitation of iron from the seas that lead to the iron rich strata now used for iron ore
- oxygenation also resulted in a radiation of greenhouse gasses such as CO2 which lead to the great cooling leading to snowball earth
what are the origins of extremophile prokaryotes
archaea
what are the 2 main biochemical cycles of life
calvin cycle and the krebs cycle - both use carbon for energy storage and transfer
evolution of what process lead to the great oxygenation event
photosynthesis
what does photosynthesis require
a means by which photons are captured and their energy directed to the reaction centre where CO2 is fixed
what is the light harvesting complex
it sits embedded in a membrane and consists of proteins and pigments surrounding a reaction centre
(various pigment molecules bound to proteins)
where is the light harvesting complex of green plants found
the thylakoid membranes of chloroplasts
what are antenna pigments
chlorophyll b, xanthophylls, carotenes
why is it good that absorption spectra are not overlapping
because when you have more than one pigment you broaden the absorption range for photosynthesis
what is the other role of carotenoids
they act as an antioxidant to prevent photo-oxidative damage of chlorophyll
each antenna complex has between 250-400 pigment molecules and the energy they absorb is shuttled to the specialized chlorophyll a proteins complex known as the .……….. …………. of the photosystem
reaction centre
how is energy shuttled in photosynthesis
by resonance energy transfer
what is a photosystem
composed of a reaction centre surrounded by several light harvesting complexes
what is a reaction centre
association of proteins holding a special pair of chlorophyll a molecules and a primary electron acceptor
nitrogen fixation by pants requires …………
microbes
what fixes atmospheric nitrogen into a more suable form such as ammonia
diazotrophsare bacteria and archae
what are the steps in the nitrogen cycle
- atmospheric nitrogen absorbed by nitrogen fixing bacteria in plant roots or taken into the grounf by decomposers
- nitrogen fixing soil bacteria result in ammonification giving ammonium
- nitrifying bacteria result in nitrification producing nitrites
- nitrates are produced form nitrites by nitrifying bacteria
- denitrifying bacteria convert nitrates int atmospheric nitrogen or nitrate assimilation occurs into plants which are eaten by animals
as far as we know all living systems derive(d) their energy from
electrons
electrons are used in chemiosmosis what is this
where electrons flow down their conc gradient and H are pumped against their conc gradient across the membrane
when H flow back down their conc gradient they pass through ATP synthase, generating ATP
give an example of another redox reaction which creates energy other than photosynthesis
NO2 reduction
archaea are more closely related to …….. than bacteria and where does this evidence come from
eukaryotes
evidence from genes sequencing - particularly rRNA
what are the different energy sources of archaea
organic resources
phototrophic
carbon dioxide fixation
archaea membranes are comprised of …… lipids not ……. lipids
ether not ester
how do archaea reproduce
asexually
have any pathogenic archaea been found
no - only mutualistic and commensals
what about archaea membranes stabilises them at high temperatures
their highly branched structure - they contain branched isoprene chains
what can isoprene chains of archaea membranes do
they can be joined together between phospholipids
they can form carbon rings which increase stability
what do methanogenic archaea do
they breakdown lignocellulose into simple sugars and methane (the by-product) - methanogens are key organisms in decomposition and they are obligate anaerobes
what are thermoacidophiles
can cope with extreme temp and pH e.g. picrophilustorridus
- thick extracellular protein mucilage
- they have an S layer
- they oxidise sugars via modified glycolysis (entner dudoroft)
- 12% of genes are transporters