Extra Flashcards
Turbidity calculations
A260* dilution factor*50ng/ul=DNA concentration
How to know if colicins present in stab plate?
Zone of inhibition around stab site
How do we know if multiple strains made same colicin?
Strains won’t inhibit the others growth
How do we know if multiple strain made different colicin?
Inhibit growth of colicin
What is a macro plaque?
A zone of complete clearing, complete cell lysis
How will we know if our cell has an F plasmid?
If present, QB infects those cells to create a high density innoculum, over 20 phages per mL
If no plasmid present, phage will not infect and final titer will be less than 20 phage/mL
What was the purpose of milk agar in caseinase experiment?
See if organism could break down casein into casamino acids
What was the purpose of our Final dilution in casein broth and our initial dilution in casein broth?
See if caseinase expression is quorum regulated
What was the purpose of the casamino broth
See if our organism can use casamino acids correctly, will it grow in it at all?
On the experimental acinetobacter agar(no trp), what will grow vs not grow?
TRP+ DNA/Trp- cells and AcDNA and trp- cells: Will grow because the trp- cell should be able to do transformation
PsDNA/Trp- cells- no growth, transformation is genus specific
Trp+ DNA/DNase/trp– no growth bc all DNA should be killed by DNASE, therefore no transformation possible.
On the control acinetobacter plate (trp- cells), what will grow vs. not grow
Trp+ cells and Ac cells- grow bc prototrophs
Ps cells- depend on independent starin
Trp- cells: no growth, strain is auxotroph, no available DNA to take up
On the control TSY (trp+) plate, what will grow vs not grow?
Trp+ DNA, Ac DNA and Ps DNA will not grow because we want tp ensure they were lysed correctly
Trp- cells: growth, can trp- cells take up trp+ DNA and grow?
Trp- cells/DNAse-Trp+ cells in agar will be killed in agar, no growth
How to calculate CFU/mL
colonies on plate1/amounted plated1/dilution factor
