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Microbiology and Parasitology (Lab) > Experiment Results Reviewer > Flashcards

Experiment Results Reviewer Flashcards

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1
Q

Gram Stain positive result?

A

Purple

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2
Q

Gram Stain negative result?

A

Red

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3
Q

Acid Fast?

A

Pink/Red

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4
Q

Non Acid Fast?

A

Blue/Purple

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5
Q

Negative Staining?

A

White MO against black stain

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6
Q

(Flagella Staining) Wet Mount Staining?

A

Dark purple MO against purple stain

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7
Q

(Flagella Staining) Leifson’s Staining?

A

Blue MO against red stain

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8
Q

(Endospore Staining) Endospore

A

Green

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9
Q

(Endospore Staining) Vegetative Cell

A

Pink

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10
Q

Capsule Staining results?

A

Purple stain, clear capsule, purple MO

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11
Q

(Carbohydrate Fermentation) Culture Media Used?

A

Lactose Broth and TSI and Agar Deep Slant Tube

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12
Q

(Carbohydrate Fermentation, broth medium) Red to red?

A

negative

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13
Q

(Carbohydrate Fermentation, broth medium) Red to cloudy red?

A

some growth but still negative

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14
Q

(Carbohydrate Fermentation, broth medium) Red to yellow?

A

positive with organic acids

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15
Q

(Carbohydrate Fermentation, broth medium) Red to yellow with bubble in Durham tube

A

positive with organic acids and gas

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16
Q

(Carbohydrate Fermentation, solid medium) Yellow deep to red slant?

A

positive with glucose fermentation

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17
Q

(Carbohydrate Fermentation, solid medium) Yellow deep to yellow slant?

A

positive with glucose, lactose, and sucrose fermentation

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18
Q

(Carbohydrate Fermentation, solid medium) Red deep to red slant?

A

negative with no sugar fermentation

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19
Q

(Methyl Red Test and Vogues-Proskauer Test) Culture Media used?

A

Methyl Red-Voges Proskauer Broth

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20
Q

(Methyl Red Test) Positive result?

A

Red

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21
Q

(Methyl Red Test) Negative result?

A

Yellow

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22
Q

(Voges-Proskauer Test) Positive result?

A

Red

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23
Q

(Voges-Proskauer Test) Negative result?

A

Yellow

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24
Q

(Voges-Proskauer Test) Negative result?

A

Yellow

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25
Q

(Citrate Utilization Test) Culture Media Used?

A

Simmons Citrate Agar (SCA) Slants

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26
Q

(Citrate Utilization Test) Positive result?

A

Blue indicates an alkaline pH

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27
Q

(Citrate Utilization Test) Negative result?

A

Green indicates a neutral pH

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28
Q

(Indole Production Test) Culture Media Used?

A

Sulfide Indole Motility (SIM)

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29
Q

(Indole Production Test) purple color of kovac’s reagent, no black, but turbid

A

indole positive, sulfide negative, motile

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30
Q

(Indole Production Test) purple color of kovac’s medium, black

A

indole positive, sulfide and motile positive

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31
Q

(Indole Production Test) yellow and has sharp line of growth

A

all negative reactions

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32
Q

(Lysine Decarboxylase Test) Culture Media Used?

A

Lysine Iron Agar (LIA) Slants

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33
Q

(Lysine Decarboxylase Test) positive result?

A

purple and has alkaline pH

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34
Q

(Lysine Decarboxylase Test) negative result?

A

yellow and has acidic pH

35
Q

(Lysine Decarboxylase Test) presence of hydrogen sulfide?

A

black

36
Q

(Phenylalanine Deamination) Culture Media Used?

A

Phenylalanine Agar Slants

37
Q

(Phenylalanine Deamination) positive result?

A

Green which means an acid is present

38
Q

(Phenylalanine Deamination) negative result?

A

Yellow and acid is not present

39
Q

(Urease Test) Culture Media Used?

A

Urea Broth

40
Q

(Urease Test) positive result?

A

bright pinkish-red is pH>8.1

41
Q

(Urease Test) negative result?

A

orange-yellow is pH<6.8

42
Q

(Catalase Test) positive result?

A

formation of bubbles

43
Q

Purple

A

Gram Stain positive result?

44
Q

Red

A

Gram Stain negative result?

45
Q

Pink/Red

A

Acid Fast?

46
Q

Blue/Purple

A

Non Acid Fast?

47
Q

White MO against black stain

A

Negative Staining?

48
Q

Dark purple MO against purple stain

A

(Flagella Staining) Wet Mount Staining?

49
Q

Blue MO against red stain

A

(Flagella Staining) Leifson’s Staining?

50
Q

Green

A

(Endospore Staining) Endospore

51
Q

Pink

A

(Endospore Staining) Vegetative Cell

52
Q

Purple stain, clear capsule, purple MO

A

Capsule Staining results?

53
Q

Lactose Broth and TSI and Agar Deep Slant Tube

A

(Carbohydrate Fermentation) Culture Media Used?

54
Q

negative

A

(Carbohydrate Fermentation, broth medium) Red to red?

55
Q

some growth but still negative

A

(Carbohydrate Fermentation, broth medium) Red to cloudy red?

56
Q

positive with organic acids

A

(Carbohydrate Fermentation, broth medium) Red to yellow?

57
Q

positive with organic acids and gas

A

(Carbohydrate Fermentation, broth medium) Red to yellow with bubble in Durham tube

58
Q

positive with glucose fermentation

A

(Carbohydrate Fermentation, solid medium) Yellow deep to red slant?

59
Q

positive with glucose, lactose, and sucrose fermentation

A

(Carbohydrate Fermentation, solid medium) Yellow deep to yellow slant?

60
Q

negative with no sugar fermentation

A

(Carbohydrate Fermentation, solid medium) Red deep to red slant?

61
Q

Methyl Red-Voges Proskauer Broth

A

(Methyl Red Test and Vogues-Proskauer Test) Culture Media used?

62
Q

Red

A

(Methyl Red Test) Positive result?

63
Q

Yellow

A

(Methyl Red Test) Negative result?

64
Q

Red

A

(Voges-Proskauer Test) Positive result?

65
Q

Yellow

A

(Voges-Proskauer Test) Negative result?

66
Q

Yellow

A

(Voges-Proskauer Test) Negative result?

67
Q

Simmons Citrate Agar (SCA) Slants

A

(Citrate Utilization Test) Culture Media Used?

68
Q

Blue indicates an alkaline pH

A

(Citrate Utilization Test) Positive result?

69
Q

Green indicates a neutral pH

A

(Citrate Utilization Test) Negative result?

70
Q

Sulfide Indole Motility (SIM)

A

(Indole Production Test) Culture Media Used?

71
Q

indole positive, sulfide negative, motile

A

(Indole Production Test) purple color of kovac’s reagent, no black, but turbid

72
Q

indole positive, sulfide and motile positive

A

(Indole Production Test) purple color of kovac’s medium, black

73
Q

all negative reactions

A

(Indole Production Test) yellow and has sharp line of growth

74
Q

Lysine Iron Agar (LIA) Slants

A

(Lysine Decarboxylase Test) Culture Media Used?

75
Q

purple and has alkaline pH

A

(Lysine Decarboxylase Test) positive result?

76
Q

yellow and has acidic pH

A

(Lysine Decarboxylase Test) negative result?

77
Q

black

A

(Lysine Decarboxylase Test) presence of hydrogen sulfide?

78
Q

Phenylalanine Agar Slants

A

(Phenylalanine Deamination) Culture Media Used?

79
Q

Green which means an acid is present

A

(Phenylalanine Deamination) positive result?

80
Q

Yellow and acid is not present

A

(Phenylalanine Deamination) negative result?

81
Q

Urea Broth

A

(Urease Test) Culture Media Used?

82
Q

bright pinkish-red is pH>8.1

A

(Urease Test) positive result?

83
Q

orange-yellow is pH<6.8

A

(Urease Test) negative result?

84
Q

formation of bubbles

A

(Catalase Test) positive result?

Microbiology and Parasitology (Lab) (6 decks)

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