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Microbiology and Parasitology (Lab) > Biochemical Activities of Bacteria > Flashcards

Biochemical Activities of Bacteria Flashcards

1
Q

Sum of all chemical reaction that occur within a living cell

A

Metabolism

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2
Q

Metabolic activity of bacteria in the presence of enzymes

A

Endoenzymes and exoenzymes

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3
Q

Energy-producing reactions of bacteria

A

Oxidative process and fermen

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4
Q

Biochemical activities

A

Carbohydrate fermentation, Organic Nitrogen Metabolism, Electron Transport

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5
Q

The substrate formed from the metabolism of carbohydrate is either glucose or lactose

A

Carbohydrate Fermentation

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6
Q

Glucose in Carbohydrate Fermentation

A

acid, alcohol, gas, organic molecule

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7
Q

Culture Media used in Fermentation in Broth Medium

A

Lactose Broth

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8
Q

(Fermentation in Broth Medium) From RED to RED

A

inability to utilize carbohydrates (negative result)

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9
Q

(Fermentation in Broth Medium) from RED to RED (cloudy)

A

Turbidity indicates growth in the medium, color unchanged indicates other possible sources of energy such as peptone (negative result)

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10
Q

(Fermentation in Broth Medium) from RED to YELLOW

A

Ability to use carbohydrates and produce organic acid (positive result)

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11
Q

(Fermentation in Broth Medium) from RED to YELLOW with bubble in Durham tube

A

Ability to use carbohydrate and produce organic acids and gas (positive result)

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12
Q

Culture Media used in Fermentation in Solid Medium?

A

TSI Agar Deep Slant Tube

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13
Q

(Fermentation in Solid Medium) indicated acid production

A

RED-YELLOW

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14
Q

(Fermentation in Solid Medium) indicates hydrogen sulfide gas

A

BLACK

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15
Q

(Fermentation in Solid Medium) YELLOW deep and RED slant

A

POSITIVE (glucose fermentation)

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16
Q

(Fermentation in Solid Medium) YELLOW deep and YELLOW slant

A

POSITIVE (indicates glucose, lactose, and sucrose fermentation)

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17
Q

(Fermentation in Solid Medium) RED deep and RED slant

A

NEGATIVE (no sugar was fermented)

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18
Q

Culture Media used with the Methyl Red Test and Voges-Proskauer Test??

A

Methyl Red-Voges Proskauer Broth (MRVP)

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19
Q

(Methyl Red Test) pH of Glucose acid?

A

4.4 and below

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20
Q

(Methyl Red Test) pH indicator?

A

Methyl red

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21
Q

(Methyl Red Test) yellow at what pH?

A

6.2

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22
Q

(Voges-Proskauer Test) Glucose —>

A

Acetoin (Acetyl Methyl Carbinol) through Butanediol fermentation

23
Q

Barritt’s A (alpha-napthol) in ethanol

24
Q

Barritt’s B (40% KOH)

25
Q

Used to differentiate between organisms that produce large amounts of acid and organisms that only produce neutral content (acetoin)

26
Q

Culture Media used in Citrate Utilization Test

A

Simmons Citrate Agar (SCA) Slants

27
Q

Indicator used in Citrate Utilization Test?

A

Bromythol blue

28
Q

Alkaline pH in Citrine Utilization Test?

29
Q

Neutral pH in Citrate Utilization Test

30
Q

Culture Media Used in Indole Production Test

A

Sulfide Indole Motility (SIM)

31
Q

Culture Media used in Lysine Decarboxylase Test

A

Lysine Iron Agar (LIA) Slants

32
Q

(Lysine Decarboxylase Test) lysine —->

A

cadaverine and CO2 through decarboxylase

33
Q

(Lysine Decarboxylase Test) Indicator used?

A

Bromcresol purple

34
Q

(Lysine Decarboxylase Test) Alkaline pH

A

Purple (positive result)

35
Q

(Lysine Decarboxylase Test) Acidic pH

36
Q

(Lysine Decarboxylase Test) H2S production

37
Q

Culture Media used for Phenylalanine Deamination

A

Phenylalanine Agar Slants

38
Q

(Phenylalanine Deamination) Phenylalanine ——>

A

phenyl pyruvic acid through phenylalanine deaminase

39
Q

(Phenylalanine Deamination) Reagent contains…

40
Q

(Phenylalanine Deamination) id acid is present it turns the medium

41
Q

(Phenylalanine Deamination) if there is negative acid present it turns the medium

42
Q

Culture Media used for Urease Test

A

Urea Broth

43
Q

(Urease Test) a byproduct of metabolism

44
Q

(Urease Test) Production of ammonia results in the _____ of the pH of the medium

45
Q

(Urease Test) Indicator used?

A

Phenol red

46
Q

(Urease Test) pH<6.8

A

orange-yellow

47
Q

(Urease Test) pH >8.1

A

bright pinkish-red

48
Q

(Catalase Test) one of the by-products of oxidation-reduction in the presence of oxygen

A

Hydrogen peroxide

49
Q

(Catalase Test) H2O2 —–>

A

water and oxygen through catalase

50
Q

(Functions of Catalase) protects bacteria from…

A

Toxic hydrogen peroxide (H2O2) accumulation which can occur during aerobic metabolism

51
Q

Anaerobic respiration involves reduction to inorganic compounds other than O2 act as final electron acceptors

A

Nitrogen Reduction Test

52
Q

(Nitrate Reduction Test) used to determine a bacterium’s ability to reduce nitrates

A

Nitrate broth

53
Q

(Nitrate Reduction Test Reagent) dimethyl-alpha-napthylamine

A

Nitrate Reagent A

54
Q

(Nitrate Reduction Test Reagent) sulfanilic acid

A

Nitrate Reagent B

Microbiology and Parasitology (Lab) (6 decks)

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