Experiment Flashcards
Why have you used this mice lineage ?
This lineage has been used in previous experiment from the research group in which they used the loxP –STOP-loxP-TdTomato construct in mouse genome to inject a transynaptic AAV within cortical area to label n+1 neurons and map them within the lumbar spinal cord.
13- Pro/Cons of the Quint Ebrain workflow
a. Pros: Semi-automated, gain of time during alignment, produce a 3D model and coordinate for each object which can be used for deeper analysis
b. Con: Requires more Random Access Memory, better PC, Pretty recent tool, Fiber segmentation takes time
What alternative we had to the Quint Ebrain workflow
a. Brain J, never managed to make it work. Fluorescence measurement but we preferred to observe the whole brain, without having to follow a pipeline for each brain section.
In which PD subtype is there a CST impairment ?
Multiple system atrophy parkinsonian variant.
What are the two mains impairments of CST in MSA PD ?
Reducted fractional anisotropy
Reduced central motor conduction time.
Cite three means to measure CST impairement involvment in humans ?
Diffusion tensor imaging
Transcranial magnetic stimulation and triple transcranial magnetic stimulation.
In mice, when does CST reaches the BS ?
At E17.
In mice, when does CST reaches the caudal medulla ?
E19.
In mice, when does CST cross the midline ?
P0
In mice, when does CST reaches the cervical segments ?
P2
In mice, when does CST reaches the thoracis segments ?
P5
In mice, when does CST reaches lumbar segments ?
P9
Cite three mains gene involved in CST specialisation.
Ctip2
Fezf2 (so they are not ending in cortical area)
Sox5 (synapse guidance and formation and growth)
marker for sensorimotor areas
BHLHB5 motor area
Important factor for CST growth
SEMA3A/C, SEMA6A
Netrin1
Pax6
Robo1/robo2
wnt, EPHA4
