EXP 9 Flashcards
What is nucleic acids?
Polymers of nucleotides
Nucleotide comprises what?
Nitrogenous base, pentose, and phosphate group
What is bonded to the pentose and via what?
nitrogenous base is bonded to the pentose via N- glycosidic bond at carbon 1, while the phosphate is bonded to the pentose via ester bond through the -OH group at carbon 3 or 5.
• Individual nucleotides are bound with
Phosphodiester bonds
Nucleic acids can be
Deoxyribonucleic acid
Ribonucleic acid
Strands for DNA & RNA
DNA - double strands
RNA - single bind
Nitrogenous base can be what?
Bicyclic purines
Monocyclic pyrimidines
What are bicyclic purines
Adenine
Guanine
Monocyclic pyrimidines for DNA and RNA
DNA
Thymine and cytosine
RNA
Uracil and cytosine
Pentose can be
Ribose and deoxyribose
DNA AND RNA CAN BE WHAT IN PENTOSE?
DNA is deoxyribose
RNA IS RIBOSE
Why nucleic acid have acidic character
acidity of the nucleic acids is caused by the phosphate residues, each of which contains H* capable of dissociation. acidity of the nucleic acids is caused by the phosphate residues, each of which contains H* capable of dissociation.
What is Polyanions
carrier of many negative charges
What is polycations
particularly with alkaline proteins (histones) - which are carriers of positive charges.
RNA is polyanion or polycation
Polyanion
Nucleates can be precipitated from the solution with what solvent?
Ethanol
Reagent for solubility of yeast RNA
Water
Ethanol
Hydrochloric acid
Sodium hydroxide
Reagent for extraction of DNA from banan
Water
Dishwashing liquid
Ice cold isopropyl alcohol
Positive result for DNA extraction
Wooly precipitate
Nucleic acids undergo what?
Hydrolysis
First stage of hydrolysis in nucleic acid
Breakdown into nucleotide
Reagent in hydrolysis
Sulfuric acid (H2SO4)
The mixture obtained after hydrolysis is called
Hydrolysate
The mixture obtained after hydrolysis is called
Hydrolysate
