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BIOCHEM > EXP 9 > Flashcards

EXP 9 Flashcards

1
Q

What is nucleic acids?

A

Polymers of nucleotides

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2
Q

Nucleotide comprises what?

A

Nitrogenous base, pentose, and phosphate group

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3
Q

What is bonded to the pentose and via what?

A

nitrogenous base is bonded to the pentose via N- glycosidic bond at carbon 1, while the phosphate is bonded to the pentose via ester bond through the -OH group at carbon 3 or 5.

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4
Q

• Individual nucleotides are bound with

A

Phosphodiester bonds

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5
Q

Nucleic acids can be

A

Deoxyribonucleic acid
Ribonucleic acid

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6
Q

Strands for DNA & RNA

A

DNA - double strands
RNA - single bind

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7
Q

Nitrogenous base can be what?

A

Bicyclic purines
Monocyclic pyrimidines

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8
Q

What are bicyclic purines

A

Adenine
Guanine

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9
Q

Monocyclic pyrimidines for DNA and RNA

A

DNA
Thymine and cytosine
RNA
Uracil and cytosine

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10
Q

Pentose can be

A

Ribose and deoxyribose

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11
Q

DNA AND RNA CAN BE WHAT IN PENTOSE?

A

DNA is deoxyribose
RNA IS RIBOSE

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12
Q

Why nucleic acid have acidic character

A

acidity of the nucleic acids is caused by the phosphate residues, each of which contains H* capable of dissociation. acidity of the nucleic acids is caused by the phosphate residues, each of which contains H* capable of dissociation.

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13
Q

What is Polyanions

A

carrier of many negative charges

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14
Q

What is polycations

A

particularly with alkaline proteins (histones) - which are carriers of positive charges.

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15
Q

RNA is polyanion or polycation

A

Polyanion

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16
Q

Nucleates can be precipitated from the solution with what solvent?

A

Ethanol

17
Q

Reagent for solubility of yeast RNA

A

Water
Ethanol
Hydrochloric acid
Sodium hydroxide

18
Q

Reagent for extraction of DNA from banan

A

Water
Dishwashing liquid
Ice cold isopropyl alcohol

19
Q

Positive result for DNA extraction

A

Wooly precipitate

20
Q

Nucleic acids undergo what?

A

Hydrolysis

21
Q

First stage of hydrolysis in nucleic acid

A

Breakdown into nucleotide

22
Q

Reagent in hydrolysis

A

Sulfuric acid (H2SO4)

23
Q

The mixture obtained after hydrolysis is called

A

Hydrolysate

24
Q

The mixture obtained after hydrolysis is called

A

Hydrolysate

25
Q

What test for nitrogenous base?

A

Test for purines

26
Q

Reagent for test for purines

A

Ammoniacal silver nitrate
(NH4OH) ammonium hydroxide

27
Q

PT for test for purines

A

White ppt

28
Q

What is test for pentose

A

Molisch test
Bials test

29
Q

Molisch reagent

A

H2SO4
A napthol
Ethanol

30
Q

Bials reagent

A

Bials reagent
Orcinol
Ferric ion

31
Q

PT for Molisch and bials test

A

Molisch - purple ring
Bials - bluish green

32
Q

Test for phosphate reagent

A

NH4OH
HNO3
H8mn2o4 (ammonium molybdate)

33
Q

PT for test for phosphate

A

Yellow precipitate

BIOCHEM (6 decks)

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