exp 7: isolation and analysis of dna from strawberry Flashcards
it is the polymers of nucleotides
nucleic acids
- linked by 3’ to 5’ phosphodiester bond
it is the building blocks of nucleic acids
nucleotides
- nitrogenous base:
puAG and pyCUT
- pentose sugar backbone
- phosphate grp
adenine, thymine, cytosine, guanine uracil
2 types of nucleic acids
DNA & RNA
it serves as the repository of
genes which carry the biological instruction for the synthesis of proteins
DNA
it is directly involved in protein synthesis
RNA
how can the nucleic acids be analyzed spectroscopically
bcs of their bases
how much light does nitrogenous base and aromatic amino acids absorbs light
nitrogenous base: 260 nm
aromatic amino acids absorbs light: 280nm
what can we estimate when calculating the ratio of the absorbance
the purity of the isolated sample
whatis the ratio of a pure DNA sample to a pure protein
1.8 : 0.6
how long should u smash your strawberries for
gently smash for 2 min
then add 30-50ml homogenizing soln
smash for another min
*avoid making too many soap bubbles while smashing
how much of ethanol should be added
it shld be added twice the amount of the filtrate
then allow the mix to stand for 3 min
how do u collect the dna from the strawberry
collect the floating dna in one direction only using a spooler
*then wash with small amt. of acetone
whre shld u dry the dna after extracting it
fume hood or over
for 10min
percent yield
mass of recovered dna / mass of strawberries x 100
add how much HCI
20ml
