exp 6: determination

Question 1

Health benefits of honey

Answer 1

Good source of carbs
Give strength and energy

Question 2

Honey’s sweetness

Answer 2

69% monosaccharides glucose and fructose
*used as a substitute to sugar

Question 3

Consuming honey is a great way in

Answer 3

Maintaining blood sugar level
Boosting performance
Glycogen restoration
Reduce muscle fatigue
Muscle recuperation

Question 4

Enzyme content in honey

Answer 4

Diastase
B-glucosidase
Catalase

Question 5

What test are we gg to use

Answer 5

Swliwanoff - ketososes and aldoses

Question 6

What does Seliwanoff’s reagent consists of

Answer 6

Resorcinol and concentrated hydrochloric acid

Question 7

It is a white crystalline

Answer 7

Resorcinol
Becomes pink when exposed to light when it is not pure

Question 8

It yields simple sugars

Answer 8

Acid hydrolysis of polysaccharides and oligosaccharides

Question 9

What color is produces when dehydrated ketones and aldoses reacts with resorcinol

Answer 9

Ketoses- Deep cherry red color
Aldoses- faint pink color

Question 10

Reagents used

Answer 10

1.0 mg/mL fructose solution - generate standard curve
Resorcinol reagent - differentiate ketose and aldose
6M HCl - hydrolysis sugars

Question 11

How many ml of serial dilution

Answer 11

2ml

Question 12

How many ml of resorcinol reagent and hydrochloric acid

Answer 12

1ml

*use serological pipette

Question 13

Heat and cool the test tubes for how long

Answer 13

Heat - 10min
Cool - 5min

Question 14

Record the absorbance of the samples at

Answer 14

520nm within 30min