exp 63

Question 1

exp 63 objective

Answer 1

to learn a variety of expos which help us ID staph pathogens

Question 2

Tests and Media for exp 63

Answer 2

Mannitol Salt test (Mannitol Salt plates)
coagulase tubes
DNSse plate
Novobiocin Sensitivity test (Mueller-Hinton Plates)

Question 3

Mannitol Salt test
purpose
results
Mannitol Fermentation

Answer 3

Media is selective for salt tolerant mod such as staphylococcal.
Positive yellow
Negative no yellow

Question 4

Coagulase test
purpose
breakdown
results

Answer 4

Production of coagulase is indicative of S.aureus strain
Fibrinogen—-fibrin
media thick—-negative
media liquid —-positive

Question 5

DNAse test
purpose
breakdown
results

Answer 5

can be used to reconfirm ID of s.aureus
DNA------nucleotides
cover media with 1% toulvidine
pink halo around mo----Positive
no halo-----negative

Question 6

Novobiocin sensitivity test results

purpose

Answer 6

to distinguish S. epidermis from S. saprophyticus.
measure zone of inhibition in mm
any size zone other than 0----S
no zone-----R
dont measure