Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

microbiology lab > Biochemical Test Part 1 > Flashcards

Biochemical Test Part 1 Flashcards

You may prefer our related Brainscape-certified flashcards:
Biology 101 flashcards
1
Q

Exp 22A Starch hydrolysis

substrate and producs

A

starch—–glycoses

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Exp 22B- Lipid hydrolysis

substrate and products

A

tributyrin—-glycerol and fatty acids

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Exp 25A Indole production
substrate and products
combined with

A

triptophan—-indole + pyruvic acid+ammonia

combined with 26

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Exp 26 Hydrogen sulfate test
substrate and products
combined with

A

thiosulfate—–sulfite+hydrogen sulfide gas

combined with 25 A

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Exp 25 B methyl red test
substrate and products
combined with

A

glucose—acid

25 C

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Exp 25 C Voges-Prokauer test
substrate and products
combined with

A

glucose—base

25 B

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Exp 25 D Citrate Utilization test

substrate and products

A

citrate—pyruvic acid+acetic acid+CO2

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Exp 27 Urease test

substrate and products

A

urea—CO2+H20+ammonia

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Exp 23 Carbohydrate Fermentation test

substrate and products

A

carb—Acid (A) or Acid gas (AG)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Exp 28 Litmus milk reaction test

substrate and products

A

milk—-7 possible results

  • Lactose fermentation
  • Gas production
  • Litmus reduction
  • curd formation
  • proteolysis
  • alkaline reaction
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Starch hydrolysis

+ and -

A

+ remains light

- dark

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Lipid Hydrolysis

+ and -

A

+ media around becomes transparent

- media stays cloudy

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q 
Indole production (25 A) combined with 26 
\+ and -
A

+ Red layer on surface of media

- No red layer in media

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

hydrogen sulfate test (26) combined with 25 A
this is done first!!
+ and -

A

+ black in media

-no black in media

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

methyl red test (25 B) combined with 25 C

+ and -

A

+ any red present in media

- no red

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Voges- Prokauer test (25 C) combined with 25 B

+ and -

A

+ and red in media

- no red in media

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

Citrate utilization test (25 D)

+ and -

A

+ any blue in media

-no blue in media

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

Urea (27)

+ and -

A

+ pink (metallic)

- peachy

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

Exp 29- Nitrate reduction test

substrate and products

A

nitrate—-nitrites—ammonia

20
Q

Exp 30 Catalase test

substrate and product

A

hydrogen peroxide (H2O2)—H20 and O2

21
Q

Exp 29- Nitrate reduction test

substrate and products

A

nitrate—-nitrites—ammonia

22
Q

Exp 30 Catalase test

substrate and product

A

hydrogen peroxide (H2O2)—H20 and O2

23
Q

Media used in exp 14: media for routine cultivation

A

Broths of salt, dextrose, nutrient and yeast extract

24
Q

Media used in temp requirement

A

Trypticase soy agar plates

25
Q

Media used for oxygen requirement

A

Brain heart melt tubes and SAB melt tube

26
Q

Starch hydrolysis media

A

Starch plate

27
Q

Lipid hydrolysis media

A

Tributyrin plate

28
Q

Carb fermentation test media

A

Broths of lactose, dextrose and sucrose

29
Q

Indole production test media

A

SIM deep

30
Q

Methyl red test and voges media

A

MRVP broths

31
Q

Citrate media

A

Citrate slants

32
Q

Urea media

A

Urea broths

33
Q

Nitrate media

A

Try process nitrate broths

34
Q

Catalase test media

A

Trypticase soy slant

35
Q

Lactose Fermentation acid

A

media is pink

broke down lactose into acid

36
Q

acid followed by reduction

A

majority white with pink band on top

lactose—acid—reduced acid

37
Q

acid reduction and curd

A

solid white at bottom, white broth middle and pink band on top.
Lactose –acid–reduced acid
casein–curd

38
Q

Acid reduction, curd and gas

A

solid bottom white, white broth in middle with fissures and pink on top
casein—curd
lactose—acid and gas
acid—reduced acid

39
Q

litmus reduction

A

white media with purple band on top
litmus–reduced
received electrons DIDNT break down anything (attached form of hydrogen)

40
Q

proteolysis

A

whey like brownish with deep purple band

casein—aminoacids

41
Q

Alkaline reaction

A

media unchanged or deep blue

lactose—base

42
Q

Nitrate reduction test +, -

A

any red in media—-POSITIVE NITRITE
red in media after zinc—-NEGATIVE, NITRATE (product)
no red in media after zinc—POSITIVE AMONIA

43
Q

Iodine Reagent

A

Starch hydrolysis

44
Q

Kovac’s

A

Indole production reagent

45
Q

hydrogen peroxide

A

Catalse test reagent

microbiology lab (9 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions