Exp 14 Media For Routine Cultivation Flashcards
Lab media used
Broths of salt, dextrose, nutrient and yeast extract
Objective of media for routine cultivation
To determine the nutritional (media) requirements of each no tested
Method used
Dropper bottle inoculation
Growth can be measured quantitatively by a
Spectrophotometer
T%
Goes right thru tube
A%
Absorbed by micro
The higher the A% the higher the
Number of mos
More turbidity
Increased growth
Spectrophotometer set at
600 nm
what did you use to disperse mo before spectrophotometer
Voltrex to disperse organisms
“control tube” with spectrophotometer
you had to blank the materia. Pressed “mode” on machine so it would subtract that number from the experiment. Didn’t press it with exp. tube.
to eliminate form reading anything that is not the mo