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MCB 101 Lab > Exercise 5_ Cultural Characteristics of Bacteria > Flashcards

Exercise 5_ Cultural Characteristics of Bacteria Flashcards

1
Q

Goal of characterization

A

establish dataset describing the properties of a prokaryotic pure culture

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2
Q

when was UV illuminator used>?

A

in the illumination the cultures inoculated in KMBA after its prolonged incubation

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3
Q

tool used to illuminate the cultures inoculated in KMBA after its prolonged incubation

A

UV illuminat

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4
Q

what control organism was streaked on YDCA

A

M luteus

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5
Q

what control organism was streaked on KMBA

A

Pseudomonas fluorescence

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6
Q

what control organism was streaked on TCZA

A

Klebsiella aerogenes

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7
Q

what cultural characteristics can be seen in agar medium

A

colony or pigmentation margin elevation surface and texture

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8
Q

colony or pigmentation, margin, elevation, surface and texture, these characteristics are seen in what medium?

A

agar medium

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9
Q

what cultural characteristics can be seen in broth medium

A

turbidity pellicle and ring formation sedimentation

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10
Q

turbidity, pellicle and ring formation, sedimentation these characteristics are seen in what medium?

A

broth medium

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11
Q

techniques employed for this exercise

A

aseptic technique streak plating slide inoculation

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12
Q

any nutrient material for growth and cultivation of microorganisms in the lab

A

culture media

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13
Q

uses of cultural media

A
  • for growth and maintenance of microbial cultures - to favor the production of particular compounds - to study microbial action on some constituents of the medium
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14
Q

cite examples of culture media according to their function or application

A

general purpose media selective media enriched media differential media

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15
Q

what components of KMBA serves as sources of energy

A

glucose dipotassium phosphate

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16
Q

an important step in accurate identification of microorganisms

A

description of a particular microorganism

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17
Q

description of a particular microorganism is an important step for

A

accurate identification of microorganisms

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18
Q

what is used to obtain consistent and reliable results for identification of microorganisms

A

pure culture

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19
Q

the simplest method of describing and differentiating bacteria

A

meticulously observing their colonies on agar medium

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20
Q

macroscopic appearances or growth characteristics of a particular culture on medium

A

cultural characteristics

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21
Q

cultural characteristics of bacteria are macroscopic or microscopic?

A

macroscopic

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22
Q

examples of cultural characteristics

A

colony color or pigmentation margin elevation surface and texture on agar medium turbidity pellicle and ring formation sedimentation in broth culture

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23
Q

colony color or pigmentation, margin elevation, surface and texture on agar medium, turbidity, pellicle and ring formation, sedimentation in broth culture are what type of characteristic found in bacteria

A

cultural characteristic

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24
Q

what 2 factors greatly affects the characteristics of the bacteria

A

components of media incubation condition

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25
3 media that are routinely used in studying the cultural characteristics of bacteria
NA NB nutrient gelatin
26
bacterial cultures that was used as control for this exercise
B. polymyxa Klebsiella aerogenes M luteus S aureus Pseudomonas flourescence
27
what culture media was utilized in this exercise
NA NB KMBA TZCA YDCA
28
KMBA Stands for
King's Medium B Agar (KMBA)
29
TCZA stands for
tetrazolium chloride agar
30
YDCA stands for
yeast dextrose agar
31
this culture media is recommended for identification of Pseudomonas species
KMBA
32
purpose of the utilization of KMBA in this exercise
promote pigment production of P. fluorescens
33
this medium promotes pigment production of P. fluorescens
KMBA
34
the absorbance we expect from the fluorescence in KMBA
366 nm
35
the fluorescence of P. fluorescens on KMBA was due to
low iron nutrients present in the media
36
what will happen to P. fluorescens if inoculated to KMBA with low iron content
will fluoresce
37
purpose of utilization of YDCA in this exercise
- observation of water insoluble pigment - assess the reaction of bacteria to the carbonate in the medium
38
- observation of water insoluble pigment - assess the reaction of bacteria to the carbonate in the medium (clearing) listed are the purpose of what medium ?
YDCA
39
what is being tested in the bacteria if we utilized YDCA as a culture medium
acid production from the utilization of carbonate
40
this medium is recommended for the differentiation of microorganisms especially yeasts based on the production of acid from glucose
YDCA
41
YDCA is recommended for the differentiation especially of what microorganism based on the production of acid from glucose
yeast
42
color pigmentation present in M luteus
yellow
43
this control organisms has yellow water insoluble pigment
M luteus
44
growth of which gram (+ or -) is slightly inhibited by TZC
gram positive
45
the purpose of utilization of TZCA in this exercise
- detect the redox potential of TTC of the unknown bacteria - detect if the culture is enzymatically active
46
which culture media helps in the detection of redox potential of redox potential of TTC of the bacteria
TCZA
47
TTC component of TCZA stands for
triphenyl tetrazolium chloride
48
indicator of the redox potential of the bacteria in agar plate
red pigmentation
49
the component in TZCA that can be reduced by live cells to triphenyl formazan
triphenyl tetrazolium chloride
50
triphenyl tetrazolium chloride is reduced by the live cells to what molecules ?
triphenyl formazan
51
what promotes the red pigmentation of cultures in TZCA
the reduced TTC which is the triphenyl formazan
52
TZCA is recommended for isolation of what species?
vibrio sp
53
Vibrio sp are the target culture for isolation in this culture media
TZCA
54
True or false: TTC is not heat sensitive and can be autoclave sterilized at 15 psi
False
55
TTC, a heat sensitive component of TZCA can be autoclave sterilized at what psi for how many minutes?
10 psi for 5 mins
56
aside from being heat sensitive, TTC is also ___ sensitive
light
57
heat and light sensitive component of TZCA
TTC
58
alternative way to sterilize TTC component of TZCA
thru filter sterilization
59
instead of autoclave sterilization this TZCA component is being filter sterilized
TTC
60
aside from TTC, what other materials cannot be autoclave sterilized?
ampicillin antibiotics Amino acids adenine etc
61
what component of YDCA settles at the bottom of the flask
calcium carbonate
62
what needs to be doneto avoid the settling of calcium carbonate at the bottom of the flask
- usage of glass beads - constant stirring/shaking - freezing/refrigeration of the plates poured with YDCA
63
materials needed in filter sterilizing the TTC
- membrane filter holder - membrane filter - dram vial - syringe - o ring (red) - foil
64
size of the pores of membrane filter to be utilized for filter sterilizing TTC
0.22 microns
65
conditions when microbial pigments are synthesized
- bacterial species are grown in appropriate growth conditions - metabolism of bacteria is impaired or under stressful conditions
66
important roles of microbial pigments
-aid in survival and adaptation (mimic the environment) -shield against UV rad and photooxidative damage -provide antibacterial and antifungal properties -prevent colonization -contribute to photosynthesis and assist in cellular energy production
67
type of microbial pigment which are confined to the interior of the cell and are referred to as intracellular pigments
water insoluble pigments
68
water insoluble pigments are also known as
intracellular pigments
69
microbial pigment that quickly permeate into the surrounding medium and are referred as extracellular pigments
water soluble pigments
70
water soluble pigments are also known as
extracellular pigments
71
floating biofilm formed at the air-liquid in static conditions
pellicle
72
environmental factors that may alter formation of pellicle
- oxygen availability - temp - osmolarity - available nutrients
73
which bacterial specimen formed the pellicle
P fluorescence

MCB 101 Lab (7 decks)

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