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MCB 101 Lab > Exercise 5_ Cultural Characteristics of Bacteria > Flashcards

Exercise 5_ Cultural Characteristics of Bacteria Flashcards

1
Q

Goal of characterization

A

establish dataset describing the properties of a prokaryotic pure culture

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2
Q

when was UV illuminator used>?

A

in the illumination the cultures inoculated in KMBA after its prolonged incubation

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3
Q

tool used to illuminate the cultures inoculated in KMBA after its prolonged incubation

A

UV illuminat

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4
Q

what control organism was streaked on YDCA

A

M luteus

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5
Q

what control organism was streaked on KMBA

A

Pseudomonas fluorescence

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6
Q

what control organism was streaked on TCZA

A

Klebsiella aerogenes

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7
Q

what cultural characteristics can be seen in agar medium

A

colony or pigmentation margin elevation surface and texture

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8
Q

colony or pigmentation, margin, elevation, surface and texture, these characteristics are seen in what medium?

A

agar medium

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9
Q

what cultural characteristics can be seen in broth medium

A

turbidity pellicle and ring formation sedimentation

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10
Q

turbidity, pellicle and ring formation, sedimentation these characteristics are seen in what medium?

A

broth medium

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11
Q

techniques employed for this exercise

A

aseptic technique streak plating slide inoculation

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12
Q

any nutrient material for growth and cultivation of microorganisms in the lab

A

culture media

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13
Q

uses of cultural media

A
  • for growth and maintenance of microbial cultures - to favor the production of particular compounds - to study microbial action on some constituents of the medium
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14
Q

cite examples of culture media according to their function or application

A

general purpose media selective media enriched media differential media

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15
Q

what components of KMBA serves as sources of energy

A

glucose dipotassium phosphate

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16
Q

an important step in accurate identification of microorganisms

A

description of a particular microorganism

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17
Q

description of a particular microorganism is an important step for

A

accurate identification of microorganisms

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18
Q

what is used to obtain consistent and reliable results for identification of microorganisms

A

pure culture

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19
Q

the simplest method of describing and differentiating bacteria

A

meticulously observing their colonies on agar medium

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20
Q

macroscopic appearances or growth characteristics of a particular culture on medium

A

cultural characteristics

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21
Q

cultural characteristics of bacteria are macroscopic or microscopic?

A

macroscopic

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22
Q

examples of cultural characteristics

A

colony color or pigmentation margin elevation surface and texture on agar medium turbidity pellicle and ring formation sedimentation in broth culture

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23
Q

colony color or pigmentation, margin elevation, surface and texture on agar medium, turbidity, pellicle and ring formation, sedimentation in broth culture are what type of characteristic found in bacteria

A

cultural characteristic

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24
Q

what 2 factors greatly affects the characteristics of the bacteria

A

components of media incubation condition

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25
Q

3 media that are routinely used in studying the cultural characteristics of bacteria

A

NA NB nutrient gelatin

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26
Q

bacterial cultures that was used as control for this exercise

A

B. polymyxa Klebsiella aerogenes M luteus S aureus Pseudomonas flourescence

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27
Q

what culture media was utilized in this exercise

A

NA NB KMBA TZCA YDCA

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28
Q

KMBA Stands for

A

King’s Medium B Agar (KMBA)

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29
Q

TCZA stands for

A

tetrazolium chloride agar

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30
Q

YDCA stands for

A

yeast dextrose agar

31
Q

this culture media is recommended for identification of Pseudomonas species

32
Q

purpose of the utilization of KMBA in this exercise

A

promote pigment production of P. fluorescens

33
Q

this medium promotes pigment production of P. fluorescens

34
Q

the absorbance we expect from the fluorescence in KMBA

35
Q

the fluorescence of P. fluorescens on KMBA was due to

A

low iron nutrients present in the media

36
Q

what will happen to P. fluorescens if inoculated to KMBA with low iron content

A

will fluoresce

37
Q

purpose of utilization of YDCA in this exercise

A
  • observation of water insoluble pigment - assess the reaction of bacteria to the carbonate in the medium
38
Q
  • observation of water insoluble pigment - assess the reaction of bacteria to the carbonate in the medium (clearing) listed are the purpose of what medium ?
39
Q

what is being tested in the bacteria if we utilized YDCA as a culture medium

A

acid production from the utilization of carbonate

40
Q

this medium is recommended for the differentiation of microorganisms especially yeasts based on the production of acid from glucose

41
Q

YDCA is recommended for the differentiation especially of what microorganism based on the production of acid from glucose

42
Q

color pigmentation present in M luteus

43
Q

this control organisms has yellow water insoluble pigment

44
Q

growth of which gram (+ or -) is slightly inhibited by TZC

A

gram positive

45
Q

the purpose of utilization of TZCA in this exercise

A
  • detect the redox potential of TTC of the unknown bacteria - detect if the culture is enzymatically active
46
Q

which culture media helps in the detection of redox potential of redox potential of TTC of the bacteria

47
Q

TTC component of TCZA stands for

A

triphenyl tetrazolium chloride

48
Q

indicator of the redox potential of the bacteria in agar plate

A

red pigmentation

49
Q

the component in TZCA that can be reduced by live cells to triphenyl formazan

A

triphenyl tetrazolium chloride

50
Q

triphenyl tetrazolium chloride is reduced by the live cells to what molecules ?

A

triphenyl formazan

51
Q

what promotes the red pigmentation of cultures in TZCA

A

the reduced TTC which is the triphenyl formazan

52
Q

TZCA is recommended for isolation of what species?

53
Q

Vibrio sp are the target culture for isolation in this culture media

54
Q

True or false: TTC is not heat sensitive and can be autoclave sterilized at 15 psi

55
Q

TTC, a heat sensitive component of TZCA can be autoclave sterilized at what psi for how many minutes?

A

10 psi for 5 mins

56
Q

aside from being heat sensitive, TTC is also ___ sensitive

57
Q

heat and light sensitive component of TZCA

58
Q

alternative way to sterilize TTC component of TZCA

A

thru filter sterilization

59
Q

instead of autoclave sterilization this TZCA component is being filter sterilized

60
Q

aside from TTC, what other materials cannot be autoclave sterilized?

A

ampicillin antibiotics Amino acids adenine etc

61
Q

what component of YDCA settles at the bottom of the flask

A

calcium carbonate

62
Q

what needs to be doneto avoid the settling of calcium carbonate at the bottom of the flask

A
  • usage of glass beads - constant stirring/shaking - freezing/refrigeration of the plates poured with YDCA
63
Q

materials needed in filter sterilizing the TTC

A
  • membrane filter holder - membrane filter - dram vial - syringe - o ring (red) - foil
64
Q

size of the pores of membrane filter to be utilized for filter sterilizing TTC

A

0.22 microns

65
Q

conditions when microbial pigments are synthesized

A
  • bacterial species are grown in appropriate growth conditions - metabolism of bacteria is impaired or under stressful conditions
66
Q

important roles of microbial pigments

A

-aid in survival and adaptation (mimic the environment) -shield against UV rad and photooxidative damage -provide antibacterial and antifungal properties -prevent colonization -contribute to photosynthesis and assist in cellular energy production

67
Q

type of microbial pigment which are confined to the interior of the cell and are referred to as intracellular pigments

A

water insoluble pigments

68
Q

water insoluble pigments are also known as

A

intracellular pigments

69
Q

microbial pigment that quickly permeate into the surrounding medium and are referred as extracellular pigments

A

water soluble pigments

70
Q

water soluble pigments are also known as

A

extracellular pigments

71
Q

floating biofilm formed at the air-liquid in static conditions

72
Q

environmental factors that may alter formation of pellicle

A
  • oxygen availability - temp - osmolarity - available nutrients
73
Q

which bacterial specimen formed the pellicle

A

P fluorescence

MCB 101 Lab (7 decks)

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