Exercise 5_ Cultural Characteristics of Bacteria Flashcards
Goal of characterization
establish dataset describing the properties of a prokaryotic pure culture
when was UV illuminator used>?
in the illumination the cultures inoculated in KMBA after its prolonged incubation
tool used to illuminate the cultures inoculated in KMBA after its prolonged incubation
UV illuminat
what control organism was streaked on YDCA
M luteus
what control organism was streaked on KMBA
Pseudomonas fluorescence
what control organism was streaked on TCZA
Klebsiella aerogenes
what cultural characteristics can be seen in agar medium
colony or pigmentation margin elevation surface and texture
colony or pigmentation, margin, elevation, surface and texture, these characteristics are seen in what medium?
agar medium
what cultural characteristics can be seen in broth medium
turbidity pellicle and ring formation sedimentation
turbidity, pellicle and ring formation, sedimentation these characteristics are seen in what medium?
broth medium
techniques employed for this exercise
aseptic technique streak plating slide inoculation
any nutrient material for growth and cultivation of microorganisms in the lab
culture media
uses of cultural media
- for growth and maintenance of microbial cultures - to favor the production of particular compounds - to study microbial action on some constituents of the medium
cite examples of culture media according to their function or application
general purpose media selective media enriched media differential media
what components of KMBA serves as sources of energy
glucose dipotassium phosphate
an important step in accurate identification of microorganisms
description of a particular microorganism
description of a particular microorganism is an important step for
accurate identification of microorganisms
what is used to obtain consistent and reliable results for identification of microorganisms
pure culture
the simplest method of describing and differentiating bacteria
meticulously observing their colonies on agar medium
macroscopic appearances or growth characteristics of a particular culture on medium
cultural characteristics
cultural characteristics of bacteria are macroscopic or microscopic?
macroscopic
examples of cultural characteristics
colony color or pigmentation margin elevation surface and texture on agar medium turbidity pellicle and ring formation sedimentation in broth culture
colony color or pigmentation, margin elevation, surface and texture on agar medium, turbidity, pellicle and ring formation, sedimentation in broth culture are what type of characteristic found in bacteria
cultural characteristic
what 2 factors greatly affects the characteristics of the bacteria
components of media incubation condition
3 media that are routinely used in studying the cultural characteristics of bacteria
NA NB nutrient gelatin
bacterial cultures that was used as control for this exercise
B. polymyxa Klebsiella aerogenes M luteus S aureus Pseudomonas flourescence
what culture media was utilized in this exercise
NA NB KMBA TZCA YDCA
KMBA Stands for
King’s Medium B Agar (KMBA)
TCZA stands for
tetrazolium chloride agar
YDCA stands for
yeast dextrose agar
this culture media is recommended for identification of Pseudomonas species
KMBA
purpose of the utilization of KMBA in this exercise
promote pigment production of P. fluorescens
this medium promotes pigment production of P. fluorescens
KMBA
the absorbance we expect from the fluorescence in KMBA
366 nm
the fluorescence of P. fluorescens on KMBA was due to
low iron nutrients present in the media
what will happen to P. fluorescens if inoculated to KMBA with low iron content
will fluoresce
purpose of utilization of YDCA in this exercise
- observation of water insoluble pigment - assess the reaction of bacteria to the carbonate in the medium
- observation of water insoluble pigment - assess the reaction of bacteria to the carbonate in the medium (clearing) listed are the purpose of what medium ?
YDCA
what is being tested in the bacteria if we utilized YDCA as a culture medium
acid production from the utilization of carbonate
this medium is recommended for the differentiation of microorganisms especially yeasts based on the production of acid from glucose
YDCA
YDCA is recommended for the differentiation especially of what microorganism based on the production of acid from glucose
yeast
color pigmentation present in M luteus
yellow
this control organisms has yellow water insoluble pigment
M luteus
growth of which gram (+ or -) is slightly inhibited by TZC
gram positive
the purpose of utilization of TZCA in this exercise
- detect the redox potential of TTC of the unknown bacteria - detect if the culture is enzymatically active
which culture media helps in the detection of redox potential of redox potential of TTC of the bacteria
TCZA
TTC component of TCZA stands for
triphenyl tetrazolium chloride
indicator of the redox potential of the bacteria in agar plate
red pigmentation
the component in TZCA that can be reduced by live cells to triphenyl formazan
triphenyl tetrazolium chloride
triphenyl tetrazolium chloride is reduced by the live cells to what molecules ?
triphenyl formazan
what promotes the red pigmentation of cultures in TZCA
the reduced TTC which is the triphenyl formazan
TZCA is recommended for isolation of what species?
vibrio sp
Vibrio sp are the target culture for isolation in this culture media
TZCA
True or false: TTC is not heat sensitive and can be autoclave sterilized at 15 psi
False
TTC, a heat sensitive component of TZCA can be autoclave sterilized at what psi for how many minutes?
10 psi for 5 mins
aside from being heat sensitive, TTC is also ___ sensitive
light
heat and light sensitive component of TZCA
TTC
alternative way to sterilize TTC component of TZCA
thru filter sterilization
instead of autoclave sterilization this TZCA component is being filter sterilized
TTC
aside from TTC, what other materials cannot be autoclave sterilized?
ampicillin antibiotics Amino acids adenine etc
what component of YDCA settles at the bottom of the flask
calcium carbonate
what needs to be doneto avoid the settling of calcium carbonate at the bottom of the flask
- usage of glass beads - constant stirring/shaking - freezing/refrigeration of the plates poured with YDCA
materials needed in filter sterilizing the TTC
- membrane filter holder - membrane filter - dram vial - syringe - o ring (red) - foil
size of the pores of membrane filter to be utilized for filter sterilizing TTC
0.22 microns
conditions when microbial pigments are synthesized
- bacterial species are grown in appropriate growth conditions - metabolism of bacteria is impaired or under stressful conditions
important roles of microbial pigments
-aid in survival and adaptation (mimic the environment) -shield against UV rad and photooxidative damage -provide antibacterial and antifungal properties -prevent colonization -contribute to photosynthesis and assist in cellular energy production
type of microbial pigment which are confined to the interior of the cell and are referred to as intracellular pigments
water insoluble pigments
water insoluble pigments are also known as
intracellular pigments
microbial pigment that quickly permeate into the surrounding medium and are referred as extracellular pigments
water soluble pigments
water soluble pigments are also known as
extracellular pigments
floating biofilm formed at the air-liquid in static conditions
pellicle
environmental factors that may alter formation of pellicle
- oxygen availability - temp - osmolarity - available nutrients
which bacterial specimen formed the pellicle
P fluorescence