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MCB 101 Lab > Exercise 3_ Morphology of Filamentous Fungi > Flashcards

Exercise 3_ Morphology of Filamentous Fungi Flashcards

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1
Q

main character for exer 3

A

filamentous fungi

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2
Q

optimal temperature on which molds will grow

A

at 25 °C

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3
Q

25 °C is the optimal temperature for growth of what? yeast or mold

A

mold

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4
Q

species of molds where dichotomous branching is present

A

Allomyces Galactomyces geotrichum

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5
Q

Allomyces and Galactomyces geotrichum are representative species with

A

dichotomous branching

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6
Q

this could either be branched or unbranched

A

hyphae

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7
Q

2 forms of hyphae

A

branched or unbranched

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8
Q

Collective form of hyphae

A

mycelia

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9
Q

Two forms of molds

A

filamentous or multicellular fungi

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10
Q

Forms of mycelium

A

septated or non septated

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11
Q

defining feature between individual cells in the mycelium when it is either septated or non septated

A

crosswalls (septa)

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12
Q

allows the exchange of cytoplasm or organelles throughout the hyphae

A

crosswalls

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13
Q

what happens in the crosswalls between the individual cells in the mycelium

A

allow exchange of cytoplasm or organelles

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14
Q

Location where the branches of septated fungi are situated

A

immediately behind a septum

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15
Q

In septated hyphae, this could be seen immediately at the back of the septum

A

branches

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16
Q

this form of hypae tends to be continuous along the whole length of the filament

A

non septated hypha

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17
Q

two results of the non septation of hypa

A

coenocytic multinucleated

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18
Q

what imparts color, texture and overall appearance to the mold colony?

A

mycelium

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19
Q

Since the hypha is a structural unit, the mycelium imparts what to the mold colony?

A

color texture overall appearance

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20
Q

When melanin pigments are present, what is the resulting color of the colonies?

A

with dark pigmentation

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21
Q

location where melanin pigments are situated

A

cell walls of molds

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22
Q

this are molds with melanin pigments in their cell wall

A

dematiaceous

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23
Q

dematiaceous are molds with what?

A

melanin pigments in their cell wall

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24
Q

with the absence of pigments in their cell wall it is described as

A

hyaline

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25
Q

hyaline coincides with molds that

A

does not produce pigments in their cell walls

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26
Q

this means transparent or translucent

A

hyaline

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27
Q

hyaline is observed as

A

transparent or translucent

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28
Q

mycelial differentiation

A

submerged aerial fertile

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29
Q

type mycelial differentiation that penetrate and anchor the thalli in the substrate and absorb the nutrients

A

submerged mycelia

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30
Q

functions of submerged mycelia

A

anchor the thalli in the substrate absorb the nutrients

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31
Q

mycelia that grow above the substra

A

aerial mycelia

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32
Q

where aerial mycelia is situated

A

above the substrate

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33
Q

reproductive structures that fertile mycelia bear

A

conidia or sporangia

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34
Q

type of mycelia that harbors conidia or sporangia

A

fertile mycelia

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35
Q

the asexual spores in molds

A

sporangiospores conidia

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36
Q

sporangiospores and conidia are what type of spores? asexual or sexual

A

asexual

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37
Q

The sac where sporangipores are formed

A

sporangium

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38
Q

The sac called sporangium houses what spores?

A

sporangiospores

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39
Q

stalks where the sporangia is attached

A

sporangiophores

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40
Q

the sporangiophores stalks are attached with?

A

sporangia

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41
Q

specialized hyphae

A

conidiophores

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42
Q

conidiophores are _____ ____ where conidia is formed

A

specialized hyphae

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43
Q

what is formed on specialized hyphae called conidiophores?

A

conidia

44
Q

small conidia

A

microconidia

45
Q

large multicellular conidia

A

macroconidia

46
Q

types of conidia (4)

A

phialospores blastoconidia arthrospores chlamydospores

47
Q

phialospores, blastoconidia, arthrospores, chlamydospores are types of?

A

conidia

48
Q

vase-shaped cells

A

phialides

49
Q

conidia are produced by vase-shaped cells called phialides

A

phialospores

50
Q

where does the conidia of phialospores produced?

A

phialides (vase-shaped cells)

51
Q

the conidia of blastoconidia is produced from

A

budding of pre existing conidia

52
Q

conidia formed by separation from preexisting hyphal cells

A

arthrospores

53
Q

h does the conidia of arthropores are formed

A

by separation from preexisting hyphal cells

54
Q

characterized by spores that are large, thick-walled, round or irregular structures formed within or on the ends of a hypha

A

chlamydospores

55
Q

conidia of chlamydospores are formed where?

A

formed within or on the ends of the hypha

56
Q

conidia are produced from budding of pre existing conidia

A

blastoconidia

57
Q

sexual reproduction in molds involves the union of what

A

compatible nuclei

58
Q

the union of compatible nuclei pertains to the

A

sexual reproduction in molds

59
Q

the product of mating between 2 different organisms or hyphae

A

sexual spores

60
Q

sexual spores of molds

A

oospores ascospores zygospores basidiospores

61
Q

oospores, ascospores, zygospores, basidiospores are what type of spores

A

sexual spores

62
Q

produced when male gametes enter a large spherical cell called oogonium and fertilized the egg within

A

oospore

63
Q

oospores are produced produced when male gametes enter a large spherical cell called ______ and fertilized the egg within

A

oogonium

64
Q

formed by union of two hyphal protrusions from neighboring hyphae of two different strains

A

zygospores

65
Q

zygospores are formed by the union of wha

A

two hyphal protrusions from neighboring hyphae of two different strains

66
Q

produced in enclosures which may be oval sacs or elongated tubes called asci

A

ascospores

67
Q

ascospores are formed inside of what structure

A

asci

68
Q

produced externally on club-shaped bodies called basidia

A

basidiospores

69
Q

basidiospores are formed on

A

club-shaped bodies called basidia

70
Q

culture media utilized for this activity

A

Czapek Dox Agar (CDA) Potato dextrose agar (PDA)

71
Q

Mold specimens utilized for this exercise

A

Fusarium Geotrichum Penicillium Rhizophus Trichoderma

72
Q

the method used to characterized selected filamentous fungi morphologically

A

slide culture method

73
Q

Slide culture method is utilized to characterize molds based on its

A

morphology

74
Q

What is prepared to be placed inside the petri plate before sterilization of the slide culture set up

A

2 toothpicks 1 glass slide 1 cover slip circular paper towel/filter paper

75
Q

Incubation period for the growth of mold colony

A

1-7 days

76
Q

During the sequential point inoculation of molds, which medium was inoculated first?

A

CDA to PDA

77
Q

Incubation period for slide culture set up

A

2-7 days

78
Q

media used for slide culture set up

A

PDA

79
Q

amount of sterile water to be used in the slide culture set up

A

until the filter or paper towel is properly mositened

80
Q

do you invert the slide culture set up?

A

No

81
Q

before mounting the glass slide from the slide culture set up, what is the very f1st thing to do?

A

wipe the moisture at the bottom part of the slide

82
Q

why is it that we invert the plate during the inoculation of molds?

A
  • to insure that only the right amount of spores will be inoculated - to minimize spore dispersal
83
Q

approximate measurement of the agar blocks to be utilized in slide culture set up

A

1cm

84
Q

alternative method that can be used to prepare slide cultures

A

drop method

85
Q

mounting medium of the wet mount of the slide culture

A

lactophenol

86
Q

how many drops of lactophenol is utilized?

A

1-2 drops

87
Q

Replacement of lactophenol as mounting medium of permanent mount is desired

A

canada balsam

88
Q

canada balsam can replace _____ as mounting medium for slide culture observation

A

lactophenol

89
Q

stolon and rhizoids are what type of structures

A

vegetative structures

90
Q

specialized vegetative structures that can be observed from slide culture set

A

stolon rhizoids etc

91
Q

type of conidia present in Geotrichum

A

arthrospore

92
Q

arthrospore can be present in what mold specimen we used?

A

Geotrichum

93
Q

type of conidia present in Penicillium

A

phialospores

94
Q

phialospores can be present in what mold specimen we used?

A

Penicillium

95
Q

conidia present in Fusarium

A

macro and microconidia

96
Q

macro and microconidia can be present in what mold specimen we used?

A

Fusarium

97
Q

Sporangiospores can be present in what mold specimen we used?

A

Rhizophus

98
Q

type of spores present in Rhizopus

A

sporangiospores

99
Q

what other structures are seen in the Rhizopus specimen we used?

A

stolon rhizoid

100
Q

stolon and rhizoid are present in what mold specimen we used?

A

Rhizopus

101
Q

branched conidiophores can be present in what mold specimen we used?

A

Trichoderma

102
Q

trichoderma is observed with ____ ____ under the microscope

A

branched conidiophores

103
Q

staining agent that is present in lactophenol

A

cotton blue

104
Q

the component of lactophenol that preserves the fungal structure

A

lactic acid

105
Q

function of phenol as a component of a mounting medium

A

serves as disinfectant that kills other living organisms

MCB 101 Lab (7 decks)

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