Exercise 24 - PCR Detection

Question 0

What is the theory behind PCR, what does it accomplish

Answer 0

Diagnostic applications of PCR include detection of antibiotic resistant strains

Could tell you the difference between MRSA and non resistant Staph. A

Question 1

What does PCR stand for

Answer 1

Polymerase chain reaction

Question 2

What is the procedure for PCR

Answer 2

Take a gram + cocci

Transfer to MSA plate if positive continue to coagulase test

If coagulase positive, move to Oxacillin plate test or polymerase chain reaction test

Question 3

What are the events of PCR

Answer 3

1. Denaturation 94 C
2. Annealing (forward primer, reverse primer)
3. Extension (copy)

Question 4

Origin and purpose of Taq DNA polymerase

Answer 4

DNA polymerase is from Thermophilus aquaticus

Taq won’t denature in high heat

Question 5

What is the purpose of primers

Answer 5

To copy the DNA

Question 6

Procedure for agarose gel electrophoresis

Answer 6

Uses micro pipettes to put in holes in the gel.

Turn on machine, DNA moves toward positive charge

Question 7

How do you. Locate the mecA gene on a finished AGE

Answer 7

Produces size 533 base pairs

Question 8

What size band identifies the mecA gene of a finished AGE

Answer 8

533bp band

Question 9

What are the advantages and disadvantages of using the PCR method instead of Kirby Bauer method

Answer 9

PCR is faster, but more expensive

Question 10

What protein is produced by the mecA gene?

What ability doe it give the Staphylococci

Answer 10

PB2 - penicillin binding protein # 2

Offers resistance to cillin antibiotics