Exercise 19 - Antibiotic Sensitivity Testing

Question 0

How are the bacteria plated on the media? Lawn?

Answer 0

Spread all over the plate with a q-tip

Or you lawn them with the metal triangle instrument

Question 1

What is the name of the media used in the Kirby Bauer method

Answer 1

Mueller-Hinton agar

Question 2

What is the general procedure of the experiment in exercise 19

Answer 2

1. Swab the entire surface of petri with a bacteria
2. Place antibiotic disks on the agar
3. After incubation, measure zones of inhibition

Question 3

Zones of Inhibition: how are the measurements taken and which metric units are the answers expressed in?

Answer 3

With a ruler measuring the diameter of zone of inhibition

mm

Question 4

Know how to read a chart of antibiotics listing the zone diameters and correctly designate the bacteria to be: R, I, S to that antibiotic

Answer 4

Pg 120

Question 5

What does R, I, S stand for

Answer 5

Resistant

Sensitive

Intermediate

Question 6

Which bacteria is the most resistant to the antibiotics?

Answer 6

Pseudomonas aerugosa

Question 7

Which bacteria was the most sensitive to antibiotics?

Answer 7

Staphylococcus aureus