Exercise 20 - The Staphylococci Flashcards
Which major tests are used to distinguish the different species of staph from each other
A-toxin
Mannitol
Coagulase
DNase
Novobiocin
Gram stain reaction, size, shape and arrangement of staph?
Purple color
Large cocci
Staphylo
Know the names, purpose, ingredients, results of all media (study the chart)
Yea
What is the biochemical pattern for Staph Aureus
Beta hemolysis (alpha toxin)
Mannitol fermentation
Coagulase production
Positive for all of these tests
Which test was used to separate S. Saprophyticus form the other species
Novobiocin
Be able to read all the media for + or - reactions
Yuh
Mannitol salt agar:
What’s its selective ingredient
What’s its deferential ingredients
What does it select for
7.5% NaCl
Mannitol and phenol red
Selects for halophiles and halotolerants
Mannitol salt agar:
What is its purpose
What does it look like if it does ferment mannitol, if it doesn’t
Test for Mannitol fermentation, differentiate staph species
+ yellow (acid changing phenol red to yellow)
- red
SM110 agar staphylococcus media:
What are its selective ingredients
What are its differential ingredients
What does it select for
7.5% NaCL
Mannitol
Halophiles and Halotolerant
SM110 agar staphylococcus media:
What is its purpose?
What will Staph A look like, and CNS?
Differentiate staph species
Staph A will be a golden color
CNS will be white/opaque color
What does CNS stand for?
Coagulase Negative Staph
Blood agar:
What are its differential ingredients
What does its enrichment select for?
Hemoglobin
Fastidious bacteria
Blood agar:
What is its purpose
What do beta, alpha, gamma look like
Hemolytic patterns used in Staph/strep ID
Beta- clear agar
Alpha- green agar
Gamma- no change
Rabbit plasma:
What type of test is this?
What are its ingredients?
Differential
Proteins for clotting
Rabbit plasma:
What is its purpose
What do positive and neg results look like
Coagulase test differentiate Staph. A vs CNS
+ clot forms solid/semisolid = Staph. A
- no clot forms, liquid = CNS species
