Exercise 1 Introduction to Laboratory tools

Question 1

Micropipette parts:

___ Button
_____ Knob
_____ Button
_____ Indicator
_____ Arm
____
____ Tip

Answer 1

Plunger Button
Volume Adjustment KNob
Tip Ejector Button
Digital Volume Indicator
Ejector Arm
Shaft
Disposable TIp

Question 2

Micropipette sizes:

P 10

P20

P100

P200

P1000

Answer 2

P 10
0.5 - 10 ul

P20
2 - 20 ul

P100
10 - 100 ul

P200
20 - 200 ul

P1000
100 - 1000 ul

Question 3

Pipette Tips

P 1000

P200

P10

Answer 3

P1000
Blue

P200
Yellow

P10
Clear

Question 4

Autoclave “self-locking”

____ steam at ____ C

Duration is between ___ to ___ minutes

Answer 4

High-pressure saturated steam

121 C

15 to 20 minutes

Question 5

Creators of the autoclave

1879

1679

Answer 5

Charles Chamberland

Denis Papin

Question 6

Autoclaves are used when decontaminating biohazardous or potentially biohazardous material or sterilizing solids and liquids.

Biohazardous items are autoclaved to inactivate pathogens like ____ (4)

Answer 6

bacteria, viruses, fungi, and spores.

Question 7

Types of autoclaves (2)

Simplest of autoclaves

Usually used for sterilization by the department

Answer 7

Pressure-cooker type autoclaves (w pressure regulator)

Digital autoclaves

Question 8

Centrifuge

_____ force

They are often used to separate items in ____ using the ____ principle

Answer 8

Centrifugal force

Suspension
Sedimentation principle

Question 9

The Centripetal acceleration causes the dense substances to go to the ___ direction (at the ___ of the tube)

Answer 9

radial direction, bottom

Question 10

Centrifuge machine parts

____ Lid

____

____ overflowing the ____

____

____

____

____

____

Answer 10

Centrifuge lid

Dirty Supply Inlet

Clean liquid overflowing the Spinning Bowl

Spinning bowl

Collected Solids

Centrifuge Body

Clean Outlet

Heavy Duty Motor

Question 11

Analytical instrument used to measure the intensity of light through wavelength

It measures ____ or ___

Answer 11

Spectrophotometer

Transmission or Apparent reflectance

Question 12

AMOUNT OF LIGHT ABSORBED by a medium is PROPORTIONAL TO THE CONCENTRATION of the absorbing material or solute present.

____ is measured by ___ also called ___

Answer 12

Beer’s Law

Wavelength, nm, lambda

Question 13

When using a spectrophotometer, you should have two ___ in a plastic rack. One blank in B and one S for the sample

Answer 13

cuvettes

Question 14

Angle for the pipette ___ to ___

Answer 14

10 to 45

Question 15

tubes used by the centrifuge machine

Answer 15

Eppendorf tubes

Question 16

Sedimentation principle parts (2)

Answer 16

Supernatant

Pellet