Activity 5 DNA extraction Flashcards

1
Q

very important molecule found in all living cells and often described as the blueprint of an organism

A

Deoxyribonucleic acid (DNA)

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2
Q

Cheek cells are easily removed

With a pure sample of DNA you can test (3)

A

Genetic disease

Forensiv evidence

Study a gene involved in cancer

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3
Q

Amount of detergent to the test tube after swirling mouth in gatorade ___

Pour until half ful

A

0.5 ml

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4
Q

Purpose of detergent (2)

A

Break up lipids like in the

Cell membrane

Nuclear membrane

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5
Q

You need to add this and let it sit for 10 minutes

When DNA is stretched out it would be __ meters long

It is a ___

A

Meat tenderizer

2-3 meters long

Protease

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6
Q

____ is applied later on for about 2 cm so the DNA clumps there

It reduces the ___ of DNA

A

Cold rubbing alcohol

Solubility

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7
Q

Gatorade is ___ and the ___ help clump together the protein fragments

A

Isotonic

salt ions

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8
Q

Where is protease found?

A

Pancreas
Stomach
Intestines

Liver
Kidneys
Muscles

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9
Q

Parts of a nucleotide (3)

A

five-carbon sugar molecule called deoxyribose

Phosphate group

Nucleobases

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10
Q

Identical among all organisms

A

Sugar-phosphate arrangement in the backbone of DNA

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11
Q

Why is Adenine paired with Thymine

and

Why is Cytosine paired with guanine?

A

Chargaff’s rule. Integral to replicate each DNA molecule

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12
Q

How many chromosomes in the human body to retain the genetic blueprint

A

46 chromosomes

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13
Q

Function of DNA (5)

A

Genetic material of living organisms

Store and transmit genetic information

Regulates gene expression

Blueprint for organism construction

Govern bodily functions

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