Activity 5 DNA extraction Flashcards
very important molecule found in all living cells and often described as the blueprint of an organism
Deoxyribonucleic acid (DNA)
Cheek cells are easily removed
With a pure sample of DNA you can test (3)
Genetic disease
Forensiv evidence
Study a gene involved in cancer
Amount of detergent to the test tube after swirling mouth in gatorade ___
Pour until half ful
0.5 ml
Purpose of detergent (2)
Break up lipids like in the
Cell membrane
Nuclear membrane
You need to add this and let it sit for 10 minutes
When DNA is stretched out it would be __ meters long
It is a ___
Meat tenderizer
2-3 meters long
Protease
____ is applied later on for about 2 cm so the DNA clumps there
It reduces the ___ of DNA
Cold rubbing alcohol
Solubility
Gatorade is ___ and the ___ help clump together the protein fragments
Isotonic
salt ions
Where is protease found?
Pancreas
Stomach
Intestines
Liver
Kidneys
Muscles
Parts of a nucleotide (3)
five-carbon sugar molecule called deoxyribose
Phosphate group
Nucleobases
Identical among all organisms
Sugar-phosphate arrangement in the backbone of DNA
Why is Adenine paired with Thymine
and
Why is Cytosine paired with guanine?
Chargaff’s rule. Integral to replicate each DNA molecule
How many chromosomes in the human body to retain the genetic blueprint
46 chromosomes
Function of DNA (5)
Genetic material of living organisms
Store and transmit genetic information
Regulates gene expression
Blueprint for organism construction
Govern bodily functions