Exam Review Unit 4 Flashcards
Nucleotides in DNA
A, T, C, G
Nucleotides in RNA
A, U, C, G
Sugar in DNA
Deoxyribose
Sugar in RNA
Ribose
Structure of DNA
Double Helix
Structure of RNA
Single Strand
Location of DNA in the cell
Nucleus
Location of RNA in the cell
Nucleus and Cytoplasm
DNA Replication:
- Occurs during S phase of interphase before mitosis and meiosis
- Happens in nucleus
- Doubles DNA so that when cell divides every cell ends up with the right amount
- Semi-conservative because the end result is half the original strand and half new
List the 5 steps of DNA replication:
- Helicase unzips DNA
- Primase makes short RNA primers for DNA polymerase to get started
- DNA polymerase brings in complementary base pairs, using the 3’ – 5’ strand as a template. Then replaces RNA primers and double checks.
- DNA ligase seals the gap.
- 2 new identical DNA strands are made.
Protein Synthesis
- Occurs all of the time because the cell is always using proteins
- uses transcription and translation
Transcription:
DNA 🡪 mRNA, occurs in nucleus
List the 4 steps of transcription
- Section of DNA (gene) is unzipped
- RNA Polymerase brings in complementary RNA base pair rules
- mRNA (messenger) strand is released to leave the nucleus
- DNA zips back up
Translation:
mRNA 🡪 protein, occurs in ribosome
List the 5 steps of translation
- mRNA attaches to ribosome
- Message is read beginning at start codon “AUG”
- tRNA bring corresponding amino acids
- Amino acids bonded together with peptide bonds
- Keeps reading the mRNA in 3’s (codons) until it reaches the “stop” codon and releases the completed protein
REPLICATE the DNA strand below:
AGCATACAAACACTTATTCATC
TCGTATGTTTGTGAATAAGTAG
TRANSCRIBE and TRANSLATE the DNA strand below (look at notes for translator):
AGCATACAAACACTTATTCATC
UCGUAUGUUUGUGAAUAAGUAG
Met – Phe – Val – Asp – Lys - stop
Gametes
Sex cells; egg and sperm; haploid; result from meiosis
Somatic Cells
Body cells; diploid; result from mitosis
Haploid
1 full set of chromosomes (n)
Diploid
2 full sets of chromosomes (2n)
Homologous Chromosomes
Similar chromosomes, one from mom and one from dad, with genes located in the same place
Sister Chromatids
Identical copies of the same chromosome that result after DNA replication
Crossing Over
Process in Prophase I of meiosis where homologous chromosome pairs swap pieces of DNA, creating new gene combinations